Figure 6
From: Neofunctionalization of a duplicate hatching enzyme gene during the evolution of teleost fishes
Digestion of fertilized egg envelopes by MfHEs. (A) SDS-PAGE patterns of fertilized egg envelope digests. Fertilized egg envelopes are extracted by SDS as a control (lane 1), and digested by rMfHE1 (lane 2), rMfHE3 (lane 3), or a mixture of rMfHE1 and rMfHE3 (lane 4). Egg envelopes after hatching are extracted by SDS (lane 5). The numbers on the right represent the sizes of the major band of digests. The numbers on the left represent the sizes of the molecular markers. (B, C) Schematic diagrams of the predicted digestion mechanism of fertilized egg envelopes by MfHE1 and MfHE3. The arrows indicate the position of the cleavage sites of MfHE1 and MfHE3, respectively. Broken line represent the predicted position of intermolecular ε-(γ-glutamyl) lysine cross-links between MfZPB and MfZPCa.