Figure 8
From: Neofunctionalization of a duplicate hatching enzyme gene during the evolution of teleost fishes
The specific activities of clade I and clade II enzymes toward synthetic peptide substrates designed from the cleavage sites at the N-terminal region of ZP proteins of Euteleostei and Otocephala. Black bars represent Euteleoste (1,2: medaka, 3,4: killifish), and gray bars represent Otocephala (5, 6, 7: zebrafish, 8, 9, 10: milkfish). The specific activities of three clade I enzymes (MfHE1, MHCE and ZHE1) and two clade II enzymes (MfHE3 and MLCE) were examined using 10 peptides, whose sequences were shown in Table 2.