Figure 5

Reconstruction of the historic geographic ranges. The map shows the distribution areas of the genus Orcula in seven geographic areas: Western Alps, Northern Calcareous Alps, Southern Calcareous Alps, Western Carpathians, Western Romanian Carpathians, Dinarides and western Black Sea region. The sampling localities are indicated by black dots. The collection sites of the outgroup specimens O. aragonica in Spain (Baetic system) and O. bulgarica in Turkey (Anatolia) are not shown in the map. The linearized molecular clock dated maximum-clade-credibility tree shows the relationships of selected mt lineages (concatenated 16S, 12S and COI sequences). Black dots indicate nodes with high posterior probabilities (see Figure). The colored symbols at the branch tips indicate the geographic origin of each haplotype. At the cladogenesis events (nodes), all alternative ancestral subdivision/inheritance scenarios with likelihoods of 10% or more are indicated, separated by an "or", together with the respective likelihoods in%. When scenarios for cladogenesis events involve two ancestral areas, the symbol for the likely ancestral area/-s is/are provided left to each of the two branches. For nodes representing major splits, node ages and 95% posterior HPD intervals are indicated (see table). A time scale in mya is given below the tree. Abbreviations of the geological epochs: C: Chattian, A: Aquitanian, B: Burdigalian, L: Langhian, S: Serravallian, T: Tortonian, M: Messinian, Z: Zanclean, P: Piacenzian, G: Gelasian and C: Calabrian.