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Table 2 AMOVA of intra- and interspecific cultivar variation

From: Variable interaction specificity and symbiont performance in Panamanian Trachymyrmex and Sericomyrmexfungus-growing ants

Source of variation

Degrees of freedom

Sum of squares

Variance component

explained variation (%)

Fixation index

P value

Complete dataset

      

Between genera

1

640.97

21.64

27

ΦCT =0.27

0.3292

Among species within genera

4

774.25

31.87

39

ΦST =0.66

< 0.0001

Within species

32

876.26

27.38

34

ΦSC =0.54

< 0.0001

Total

37

2291.48

80.89

100

  

T. cornetzi sp. 1–3 and T. zeteki

      

Among species within genera

3

330.22

12.18

17

ΦST =0.17

0.0645

Within species*

15

875.36

58.35

83

  

Total

18

1205.58

70.53

100

  
  1. Results of AMOVA of Leucoagaricus symbiont diversity of the full dataset with three hierarchical levels and the second analysis considering only the T. cornetzi and T. zeteki species whose fungal symbionts were variable enough in their ITS sequences to represent different species (Figure 1). Significances are based on 10100 permutations evaluating whether fixation indices were different from a null distribution of variance parameters assuming samples were drawn from randomly chosen species.
  2. *Only a single null distribution, assuming samples randomly drawn from within species, was generated, which precluded a permutation-based significance test for this level in the two-level pairwise AMOVA.