Variant | O1 | O2 | O3 | O4 | O1 > O2 | O1 > O3 | O4 > O2 | O1 > O4 |
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CG | 7.0 × 10−3 | 2.3 × 10−7 | 5.5 × 10−3 | 5.5 × 10−5 | yes | yes | yes | yes |
PV | 6.9 × 10−3 | < 10−8 | 1.0 × 10−3 | < 10−8 | yes | yes | N.D. | yes |
- All values given in units of min−1. For the PV, yields under the O2 and O4 conditions were too small to estimate rate constants. Rank-ordering of the conditions are as follows. CG: O1 > O3 > O4 > O2; PV: O1 > O3 > O4 & O2. However, the conditions of enzyme excess are not representative of an evolutionary interpretive scenario, hence the data in Table 1 were used for this purpose. N.D. = not determined.