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Table 2 Rough estimates of k cat values under conditions of enzyme excess

From: Empirical demonstration of environmental sensing in catalytic RNA: evolution of interpretive behavior at the origins of life

Variant

O1

O2

O3

O4

O1  > O2

O1  > O3

O4  > O2

O1 > O4

CG

7.0 × 10−3

2.3 × 10−7

5.5 × 10−3

5.5 × 10−5

yes

yes

yes

yes

PV

6.9 × 10−3

< 10−8

1.0 × 10−3

< 10−8

yes

yes

N.D.

yes

  1. All values given in units of min−1. For the PV, yields under the O2 and O4 conditions were too small to estimate rate constants. Rank-ordering of the conditions are as follows. CG: O1 > O3 > O4 > O2; PV: O1 > O3 > O4 & O2. However, the conditions of enzyme excess are not representative of an evolutionary interpretive scenario, hence the data in Table 1 were used for this purpose. N.D. = not determined.