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Figure 3 | BMC Evolutionary Biology

Figure 3

From: Directed evolution of cell size in Escherichia coli

Figure 3

Evolution process and final populations of evolved lineages. The trajectory of the cell concentration (A), the mean cell size (B) and the standard deviation (C) under the size selection process. Open diamond, open circle and closed circle represent the Svr-, Mld- and T-lineages, respectively. (D) Cell size distributions of the population of the final round (left) and the isolated 12 clones (right), where the isolates are distinguished by gray-scaled lines. The top two panels represent ancestral clones. The other panels correspond to the T-, Svr- and Mld- lineages at the bottom. The insets represent the Cell IDs in Table 1. All data were obtained at approximately 107 cells/ml. The dotted line indicates one of the AC isolated clones (top left). (E) Phase contrast images of the isolated clones are shown. The insets also represent the Cell IDs in Table 1. The white bars indicate 10 μm. (F) The mean cell size of the 12 isolates. (G) The standard deviation in cell size of the 12 isolates. The error bars are 95% confidence limits. P-values are for t-test (N = 12).

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