Figure 4

The cell concentration dependency of cell size. (A) The reference lines of the cell size distribution. The dark gray, light gray and open symbols indicate the bottom 1%, mean and top 1% of the size distributions, respectively. (B) These representative images plot three references (bottom, mean and top) over cell concentrations. The left two panels represent the size distributions for an ancestral clone obtained at different cell concentrations in the same growth culture; the concentrations are indicated in the inset. The three references are plotted in the right panel along with the corresponding cell concentrations, as indicated by the arrows. (C) Shown are the references of the cell size distributions over different cell concentrations for the isolated 12 clones from the ancestor (far left), T-lineage (2nd from the left), Svr-lineage (2nd from the right) and Mld-lineage (far right) populations. Each panel includes the distribution of ACs (circle), while the other clones are indicated by squares.