Phylogeny of seven Bulinus species originating from endemic areas in three African countries, in relation to the human blood fluke Schistosoma haematobium

Table 5 Primers used for PCR amplification and sequencing

Primer name	Primer sequence	Forward or reverse	Source
Cytochrome oxidase subunit 1
CO1 (LCO490)	5’-GGT CAA CAA ATC ATA AAG ATA TTG G-3’	Forward	Folmer et al., [27]
CO2 (HCO2198)	5’-TAA ACT TCA GGG TGA CCA AAA AAT CA-3’	Reverse	Folmer et al., [27]
Asmit 1 (ATI)	5’-TTT TTT GGG CAT CCT GAG GTT TAT-3’	Forward	Bowles et al., [28]
Asmit 2 (AT2)	5’-TAA AGA AAG AAC ATA ATG AAA ATG-3’	Reverse	Bowles et al., [28]
Internal transcribed spacer of the ribosomal gene complex
ETTS1	5’- TGC TTA AGT TCA GCG GGT -3’	Reverse	Kane and Rollinson, [29]
ETTS2	5’- TAA CAA GGT TTC CGT AGG TGA A -3’	Forward	Kane and Rollinson, [29]
Small Subunit rRNA (SSU)
18SBaso3F	5’-GTG CTC TTC NCT GAG GGT CC-3’	Forward	Jørgensen et al., 2011 [8]
18SBaso9R	5’-TAC GGA AGC CTT GTT ACG A-3’	Reverse	Jørgensen et al., 2011 [8]
Large Subunit rRNA (LSU)
Baso2600F	5’-GGA ATC CGA CTG TCT AAT TAA AAC-3’	Forward	Jørgensen et al., 2011 [8]
Baso3600R	5’-CRG ATG GAT GGT AGC YTC GCA CC-3’	Reverse	Jørgensen et al., 2011 [8]

ISSN: 2730-7182