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Table 5 Primers used for PCR amplification and sequencing

From: Phylogeny of seven Bulinus species originating from endemic areas in three African countries, in relation to the human blood fluke Schistosoma haematobium

Primer name Primer sequence Forward or reverse Source
Cytochrome oxidase subunit 1
CO1 (LCO490) 5’-GGT CAA CAA ATC ATA AAG ATA TTG G-3’ Forward Folmer et al., [27]
CO2 (HCO2198) 5’-TAA ACT TCA GGG TGA CCA AAA AAT CA-3’ Reverse
Asmit 1 (ATI) 5’-TTT TTT GGG CAT CCT GAG GTT TAT-3’ Forward Bowles et al., [28]
Asmit 2 (AT2) 5’-TAA AGA AAG AAC ATA ATG AAA ATG-3’ Reverse
Internal transcribed spacer of the ribosomal gene complex
ETTS1 5’- TGC TTA AGT TCA GCG GGT -3’ Reverse Kane and Rollinson, [29]
ETTS2 5’- TAA CAA GGT TTC CGT AGG TGA A -3’ Forward
Small Subunit rRNA (SSU)
18SBaso3F 5’-GTG CTC TTC NCT GAG GGT CC-3’ Forward Jørgensen et al., 2011 [8]
18SBaso9R 5’-TAC GGA AGC CTT GTT ACG A-3’ Reverse
Large Subunit rRNA (LSU)
Baso2600F 5’-GGA ATC CGA CTG TCT AAT TAA AAC-3’ Forward Jørgensen et al., 2011 [8]
Baso3600R 5’-CRG ATG GAT GGT AGC YTC GCA CC-3’ Reverse