- Research article
- Open Access
Lack of evolutionary adjustment to ambient temperature in highly specialized cave beetles
© Rizzo et al.; licensee BioMed Central. 2015
Received: 5 September 2014
Accepted: 15 January 2015
Published: 4 February 2015
A key question in evolutionary biology is the relationship between species traits and their habitats. Caves offer an ideal model to test the adjustment of species to their surrounding temperature, as they provide homogeneous and simple environments. We compared two species living under different thermal conditions within a lineage of Pyrenean beetles highly modified for the subterranean life since the Miocene. One, Troglocharinus fonti, is found in caves at 4-11°C in the ancestral Pyrenean range. The second, T. ferreri, inhabits the coastal area of Catalonia since the early Pliocene, and lives at 14-16°C.
We found no differences in their short term upper thermal limit (ca. 50°C), similar to that of most organisms, or their lower thermal limit (ca. -2.5°C), higher than for most temperate insects and suggesting the absence of cryoprotectants. In longer term tests (7 days) survival between 6-20°C was almost 100% for both species plus two outgroups of the same lineage, but all four died between 23-25°C, without significant differences between them.
Our results suggest that species in this lineage have lost some of the thermoregulatory mechanisms common in temperate insects, as their inferred default tolerance range is larger than the thermal variation experienced through their whole evolutionary history.
One of the main questions in evolutionary biology is to link the phenotype of an organism to the environment in which it lives. This link is usually masked by the complexity of both the environmental factors that may influence an organism and its response to these factors [1,2], posing a major challenge to the understanding of the origin of phenotypic traits. Under natural conditions species are exposed to continuously changing and interacting climatic factors , to which they can adjust by behavioural plasticity, change of microhabitat or by migration [2,4].
The deep subterranean environment seems an ideal scenario to study the evolutionary adjustment of the species to their environments, as many of these confounding factors are completely absent from it. In the deepest parts of the caves and their surrounding network of fissures the conditions of the habitat are extremely constant and homogeneous, with a permanent darkness and nearly constant temperature and humidity [5,6]. Subterranean organisms have a very limited range of options to behaviourally exploit local climatic heterogeneity –as there is virtually none–, and their general lack of mobility [5,6] also reduces the possibility of migration when conditions become unfavourable. In addition, the general scarcity of resources imposes stringent requirements on the species living in this environment, resulting in simple, low diverse communities [7,8].
Caves have a homogeneous and constant temperature through the year, approximately equal to the average annual temperature of the surface [5,9], but for any given lineage of subterranean organisms this temperature may vary both geographically (including altitudinal changes) and temporally (past climatic change). It could be expected that given the harsh conditions and the stability of the environment species should have fine-tuned their thermal tolerance to the temperatures of their habitats [10-12]. For example, in a similarly constant and homogeneous environment, the cold Antarctic waters, the most stenotherm species cannot survive an increase of 2-5°C above the temperature they currently experience (below 2°C) . Cave organisms are thus an ideal model to test the long term evolutionary adjustment of organisms to the temperature of their habitats , although there is very limited information available on their comparative thermal tolerances (see Discussion).
The Pyrenean chain in the Iberian peninsula is one of the world hotspots of subterranean diversity [15,16]. Two monophyletic radiations of beetles are particularly rich, with hundreds of narrow endemic species: Trechini [17,18] (Carabidae) and Leptodirini [19,20] (Leiodidae). Within the Pyrenean Leptodirini, there was a single colonisation of the subterranean environment in the Early Miocene, leading to a radiation of more than 140 subterranean species distributed through the chain . Only in one occasion these species expanded their range outside the Pyrenees, with the colonisation of coastal areas of Catalonia during the early Pliocene by a member of the genus Troglocharinus that subsequently diversified in isolated karstic areas . The caves occupied by the species in the coastal area are on average 5°C warmer than those occupied by the species of the ancestral range in the Pyrenees (average temperature of the caves in the coast 14.2 ± 1.0°C; in the Pyrenees 8.9 ± 1.9°C), offering a unique opportunity to study the long term evolutionary adjustment of the species to their surrounding temperatures and the possible persistence of regulatory or acclimation mechanisms.
Using an experimental approach we aim to study the evolutionary adjustment of cave species to their surrounding temperatures by testing 1) if there is any significant difference in the short term thermal limits between the species living in the coast (T. ferreri) and the species living in the Pyrenees (T. fonti); 2) if there is any difference among the studied species in their longer term (up to 7 days) thermal tolerance at a range of subcritical temperatures; and 3) if there is any sign of acclimation to changing temperatures within their tolerance ranges.
The combination of an experimental approach with data on the current and past temperatures allows a better understanding of the long term evolutionary dynamics of the thermal biology of subterranean species, and its potential role in their geographical expansion and diversification. The knowledge of their thermal tolerances should also be of interest for the conservation prospects of these species, given their limited possibilities to track suitable conditions when confronting global change.
Short term experiments
Caves from which the studied specimens were collected
Range T current
Range T LGM
Range T LIG
Cova de Coll Verdaguer, Vallirana
Cova d’Ormini, Montanisell
Cova de St Salvador, Bonansa
Gouffre du Béguet, Juzet d’Izaut
Specimens of T. fonti were collected by hand in Cova d’Ormini (Pyrenees), at a temperature of 6-8°C (Figure 1; Table 1). Specimens were handled and stored in the same conditions as those used for T. ferreri, with the only exception of the temperature in the portable fridge, set to ca. 7°C.
To obtain an estimate of the average current temperature of the caves and associated subterranean environment in which the species are found we used the mean annual temperature at a 0.08 degree spatial resolution from WORLDCLIM, version 1.3 (www.worldclim.org ). The deep subterranean environment is known to have a constant temperature approximately equal to the average annual temperature of the surface [5,9]. To estimate the temperature during the Last Glacial Maximum (LGM; 21,000 YBP) we used a simulation of the general circulation model (GCM) from the Community Climate System Model (CCSM, http://www2.cesm.ucar.edu/ ). The original GCM data were downloaded from the PMIP2 website (https://pmip2.lsce.ipsl.fr/). For the Last Interglacial (LIG; 120,000-140,000 YBP) we used the data provided by , available at www.worldclim.org (Table 1).
For the experiments we used a FLIR SC305 infrared camera (FLIR Systems, Portland, US), with a resolution of 320x240 pixels and sensitivity <0.05°C at 30°C. The camera was connected to a computer using the ThermaCam Researcher Professional 2.10 software (FLIR Systems). Beetles were video taped in a climatic chamber BINDER MK53 (BINDER, Tuttlingen, Germany; range −50° to 180°C, ±0.3°C). During the experiments, specimens were fixed on their elytra with paper glue or bee wax.
For the upper thermal limit (UTL) we set up an increasing ramping program with the same general conditions as before. Upper thermal limit (i.e. the lethal temperature) was considered to be the point at which specimens stopped moving for more than a minute, as recorded by a video camera and observed through a visor by two different observers (e.g. ). Experiments were stopped at 60°C. We did not attempt to measure the critical thermal maximum (CTmax) as with glued specimens it was difficult to estimate the point at which they loose control of their movements. In the long term experiments it was also not possible to determine the CTmax (see below), the UTL being thus the only comparable measure between the two types of experiment.
For Troglocharinus fonti (the Pyrenean species) we did two replicates of 12 specimens for each the UTL and the LTL. For T. ferreri (the coastal species), and due to the limited availability of specimens, we did a single experiment of 12 specimens each for UTL and LTL.
Long term experiments
To have an estimation of thermal tolerances at more ecologically relevant conditions we set up a series of experiments using survival at longer times and temperature increases at slower rates. We used the same two species as before (the coastal Troglocharinus ferreri and the Pyrenean T. fonti), plus two outgroups of the same lineage to establish the polarity of any potential change between the two Troglocharinus species. The species used as outgroups were Trapezodirus arcticollis and Macharoscelis infernus, both from the Pyrenees (see Figure 1 and Table 1 for the location and conditions of the caves; and ref.  for the phylogenetic relationships and divergence times between the four species).
Specimens were collected by hand in the caves and transferred to the laboratory under controlled conditions, with substratum from the cave and some moss to keep humidity. In the laboratory, specimens were acclimated and stored previous to the experiments at the approximate temperature of the cave (see Table 1) in a Liebherr WTes 4177 cabinet (Bulle, Switzerland), which can establish a continuous gradient of temperature between 5-18°C, in closed plastic containers (30x21x6.5 cm) with moss and substratum from the caves (the “stock”). Specimens were kept in these conditions, considered to be closer to the natural conditions in the cave, without being disturbed until used for the experiments. For the experiments, specimens were placed in smaller plastic boxes (13x8x5.5 cm) with a 0.5 cm white plaster substratum, ca. 10 volcanic stones of a diameter of 1–3 cm saturated with distilled water, and white tissue paper also saturated in distilled water. Specimens in the experimental boxes were fed ad libitum with freshly frozen Drosophila melanogaster. Some of the control specimens were kept in the same experimental boxes for more than nine months after the end of the experiments, indicating that the observed mortality was mostly due to the temperature treatments and not to stressing conditions or a reduced longevity (which in any case would have affected both control and treatment specimens equally).
For the experiments, the box with the specimens to be treated (usually replicas of 10 individuals) was placed in a Nüve TK120 (Ankara, Turkey) climatic test cabinet (temperature range −10°C/+60°C, relative humidity, RH, range 20-95%). Controls were kept in the cabinet at the approximate temperature of the cave and RH close to saturation. The actual temperature and RH, or temperature only, inside the experimental boxes were recorded every 30" during the experiments with data loggers Testo (Lenzkirch, Germany) 176H1 (T ± 0.2°C, RH ±0.1%) or Testo 176 T4 (T ± 0.5°C, with Cu-C termopars) (Additional file 1: Table S1). Specimens were checked every 24 h, the stones and the tissue wetted and fresh food added whenever necessary. Specimens were recorded as alive when they were capable of some movement or dead when there was no movement after being poked at. We did not attempt the potential recovery of specimens apparently moving uncoordinatedly, as all life specimens were immediately returned to the incubator to minimise disturbance of the experimental conditions. No specimen considered to be dead recovered after being retired from the experimental boxes.
Experiments were conducted in replicas of 10 specimens each, including a control kept in the same conditions at the approximate temperature of the cave (12°C for T. ferreri; 7°C for T. fonti; 6°C for T. articollis and 10°C for M. infernus; Additional file 1: Table S1), and with specimens taken directly from the stock. When the number of specimens was insufficient we used smaller replicas, and when there was no mortality the same control could be re-used for successive experiments, occasionally with less than ten individuals (Additional file 1: Table S2). We conducted three types of experiments: 1) Survival at different temperatures: treated specimens were placed directly from the stock to a set temperature (6, 20, 23 or 25°C, plus controls) and survival recorded for up to 7 days. 2) Acclimation at ramping temperatures: starting at 20°C, the temperature was raised 1°C every 48 h, until all specimens died. 3) Acclimation at fixed temperatures: specimens were acclimated for seven days at 6 or 12°C and then transferred to 23°C (Additional file 1: Table S2).
For the comparison of the results of the short term experiments we used non-parametric statistical tests (Mann–Whitney U-test), as the dependent variable does not follow a normal distribution. We nonetheless computed parametric t-tests for comparison and to test the robustness of the results. For the long term experiments we also used non-parametric Mann–Whitney U-tests and Kruskal-Wallis to compare the survival at different temperatures, with multiple comparison tests to detect significant differences between means. Similarly, despite the non-normality of residuals we analysed the data through the use of generalized linear models (GLM) assuming a Poisson distribution and with post-hoc tests using the Bonferroni correction to identify significant differences among groups. All statistical analyses were conducted using SPSS 15.0.1 and Statistica 8.0.
Short term experiments
When measured using a fast temperature increase (1°C/m) the average upper thermal limit (UTL) of the specimens tested was around 50°C, with no significant differences between the species in the Pyrenees (Troglocharinus fonti, UTL = 50.9°C, sd = 0.59) and the coastal region (T. ferreri, UTL = 50.7°C, sd = 0.49) (M-W U-test: U = 75.5, p = 0.06; t-test: t-value = 0.90, d.f. = 32, p = 0.37; Figure 2B and Additional file 1: Table S2A).
Using the same speed of temperature change, the average lower thermal limit (LTL) was again not significantly different between species (T. ferreri, LTL = −2.4°C, sd = 0.91; T. fonti, LTL = −2.1°C, sd 1.21; M-W U-test: U = 109, p = 0.31; t-test: t-value = 0.75, d.f. = 33; p = 0.46; Figure 2C and Additional file 1: Table S2A). The increase in temperature due to the release of the latent heat when the tissue froze at the supercooling point was around 1°C for both species, and the temperature of the specimens took on average ca. 2 minutes to match again the ambient temperature (Figure 2A and Additional file 1: Table S2A). The decrease in body temperature was perfectly linear for both species, in synchrony with the decrease of ambient temperature, with the only interruption of the release of the latent heat (Figure 2A). There was thus no indication of temperature compensation or regulation in any of the two species tested.
Long term experiments
For the long term experiments results using non-parametric Kruskal-Wallis ANOVA (K-W), t-tests and GLM were very similar, so the latter are reported only when there were differences (see Additional file 1: Table S3 for a full account of GLM results).
Survival at different temperatures
Acclimation at ramping temperatures
Results of the acclimation experiments
T. ferreri (6 to 23°C)
T. ferreri (12 to 23°C)*
T. fonti (6 to 23°C)
T. fonti (7 to 23°C)*
T. fonti (12 to 23°C)
Acclimation at fixed temperatures
There were no significant differences in survival at 23°C between the specimens acclimated at the control temperatures and individuals acclimated for one week at a different temperature (6°C for T. ferreri, M-W U-test, U = 67.5, p = 0.49; 6 and 12°C for T. fonti, K-W test: H (2, N = 56) = 5.92, p = 0.052) (Table 2B and Additional file 1: Table S2).
The homogeneity and stability of the deep subterranean environment, both at ecological and evolutionary scales, would lead to the expectation of a close match between the thermal tolerance of the species living in it and the surrounding temperatures [10,11]. This expectation was only partially fulfilled. The maximum viable temperature range for all studied species (between close to 0°C to 23°C) was much narrower than that of non subterranean insects living at the same latitude, indicating that subterranean species in this lineage do have a modified thermal biology. However, there were no significant differences between species living in caves at substantial different temperatures.
Thus, the supercooling point of the species of Troglocharinus living in the Pyrenees and the one living in the coastal area were the same (ca. -2.5°C), and higher than that of most species of temperate invertebrates [26,28], including other species of surface Coleoptera (see e.g. [27,29-32] for measures obtained using the same methods). The supercooling point of the studied subterranean species is probably close to the freezing temperature of hemolymph without the presence of additional osmoregulation or antifreezing substances, which has been estimated at −2.79°C for a hemolymph osmolality of 1.5 osmoles .
Some subterranean Leptodirini of the same Pyrenean lineage are known to live in caves with permanent snow and ice at ca. 2-3°C, such as some species of Paratroglophyes, or Speonomites kryophilos (Fresneda & Hernando) (:page 571, ). However, due to the stability of the subterranean environment this temperature is maintained around the same value through the year, without substantial fluctuations and not reaching sub-zero temperatures. Caves at below zero temperatures are likely to be uninhabitable as water should not be available in liquid form at any time of the year. This suggests that in this lineage of cave beetles species have lost the capability to sustain low supercooling temperatures, thus avoiding the metabolic cost of synthesizing the cryoprotectants usually present in temperate insects [25,26].
We did not find differences between species in the upper thermal limits when measured in a short term ramping experiment, which in this case were similar to those found in other beetles of the same latitude (around 40-50°C, e.g. [27,29-32]), and similar to that of most eukaryotic species . It seems likely that, with the exception of species living in extreme environments such as deep oceanic vents or hydrothermal sources, most short term measures of upper thermal tolerance can only be related to general biochemical or physiological constraints, and in particular to the denaturation temperature of most proteins . The lack of adjustment of the short term measure of the UTL with local conditions seems to be a common pattern in terrestrial animals (e.g. [36-38]), and its ecological or evolutionary interpretation (e.g. ) may thus be debatable.
In sharp contrast to the estimated short term UTL, none of the species tested could survive more than 24 h at 25°C, and the survival at 23°C was also limited to 2–3 days, unlike most surface terrestrial species in temperate regions. Data on the development of some species of the same evolutionary lineage support this strict thermal limitation, with 100% egg mortality between 15-21°C . The proximate cause of mortality of terrestrial animals at subcritical temperatures (i.e. pejus temperatures in the sense of ), and specially the role of oxygen deprivation, is controversial [42-44]. For subterranean species oxygen accessibility is complicated by the water saturated atmosphere in the cave and the increased permeability of their integument, which may blur the distinction between aquatic and terrestrial habits (as in some species of “amphibious” Leptodirini, e.g. [45,46]). In any case, it seems likely that given the narrow range of tolerated temperatures and the high mortality (100% in less than 24 h at 25°C) temperature limitation must be the result of a strong physiological constraint rather than the cumulative effect of an increased metabolic rate.
Contrary to expectations, we did not find differences in the long term thermal tolerance of the Pyrenean and the coastal species. In our tests, at 6 and 20°C the survival of the adults was in all species close to 100% at seven days. Although this time may be insufficient to assess the long term effect of subcritical (pejus) temperatures, it excludes the possibility of marked differences between the coastal and Pyrenean species of Troglocharinus, as 20°C is above the temperature experienced by both species in their recent evolutionary history, and 6°C is below that experienced by the coastal species since their range expansion in the early Pliocene  (see below and Table 1). We did not find clear signs of acclimation, specially in the two Troglocharinus species tested. Some results suggest the possibility of some differences in acclimation to slow ramping temperatures with respect to the most distantly related outgroup (Macharoscelis infernus), but in any case these differences were limited to a longer survival at intermediate temperatures, maintaining the same absolute thermal limits. Some of the developmental data available also suggest the possibility of long term acclimation, as the optimal temperatures for larval development are reported to vary depending on the temperature at which the parents were reared –but again never reaching beyond the same absolute thermal limits .
All evidence thus suggest that species in this lineage of Pyrenean subterranean beetles have lost some of the physiological mechanisms linked to thermal tolerance commonly present in temperate insects, and it can be hypothesized that they reflect the default limits inherited from their most closely related surface relatives in the early Miocene (Figure 1). This does not seem to be the case for other lineages of subterranean Leptodirini in different geographical areas, such as the genus Neobathyscia in northeast Italy. Some species in this genus have retained resistance to sub-zero temperatures even if they do not experience them in their current habitat, with the freezing temperature (also measured as the release of latent heat at the supercooling point) between −6°C and −8°C in species living at different depths in the cave . They also have a better tolerance to higher temperatures  and have been shown to retain an HSP70 heat shock response, which also differs between species according to their current habitat . It is likely that Neobathyscia derives from surface ancestors that diverged from the Pyrenean lineage at least since the middle Eocene [19,20], but its precise phylogenetic position and the age of the colonization of the subterranean environment are unknown. In this case, the retention of a wider tolerance can be linked to the current habitat (with the species living closer to the entrance having a wider tolerance), and possibly to a more recent origin of the strictly subterranean adaptations .
Other subterranean organisms have been shown to have thermal tolerances broader than the extremely narrow range of temperatures of their current environment, such as e.g. some European stygobiont Crustaceans [49,50]. Although some species were sensitive to changes of only 2°C above or below their habitat temperature, others were able to tolerate without problems changes of more than 10°C . However, in this case the wider thermal tolerance was interpreted to be the result of the changes in temperature experienced during the Pleistocene , while in the species of Troglocharinus the tolerance goes beyond the temperatures experienced through their entire evolutionary history. The highest temperatures currently experienced by the species of the genus are those in the coastal area of Catalonia, with a maximum of ca. 16°C in T. ferreri. During the Miocene thermal optimum (16–14 Ma), with temperatures up to 3-4°C higher that at present , there were no Leptodirini in the coastal area, as the colonization occurred during the early Pliocene . In the Pyrenean range, an increase of 3-4°C would still maintain the temperature of the caves below 20°C. Through the Pleistocene glacial periods the Pyrenean caves currently inhabited by Leptodirini suffered a decrease of 4-5°C (Table 1) but still maintained temperatures above zero, with only very few exceptions of caves assumed to have been recently colonized. Thus, a default tolerance range between temperatures close to the freezing point of water and ca. 20°C may have sufficed these beetles for the last 30 Million years, allowing them to dispense of any costly regulatory mechanism. If this were the case, some of the coastal populations of Troglocharinus may be now close to their physiological limit, which added to their reduced mobility and habitat confinement increases the concerns on their long term survival.
In this manuscript we provide the first data on the thermal tolerance of terrestrial organisms strictly restricted to the deep subterranean environment, an ideal scenario for testing the long term adjustment of organisms to their environments. We hypothesise that these species have lost some of the physiological mechanisms related to thermal tolerance due to their likely metabolic cost in a stable environment but with severe resource restrictions. This has been possible because the "default" range (i.e. the thermal tolerance without added resistance mechanisms) was enough to allow them to survive in the constant environment of the caves through their evolutionary history. There are two main conclusions that could be inferred from our work: 1) even under what seem ideal conditions to expect a fine adjustment to temperature, other factors (in this case possibly the scarcity of resources) can override this adjustment, so that different taxa under different conditions may have similar broad tolerances; and 2) this should be a strong cautionary warning for studies trying to infer physiological tolerances from characteristics of the environment only, specially considering that in most cases organisms have many different ways to use the temporal and spatial heterogeneity of the habitat to accommodate their needs.
Availability of supporting data
All raw data are included in the Supplementary files.
We thank Charles Bourdeau, Hervé Brustel, Jordi Comas, Olivier Courtin, Arnaud Faille and Josep Pastor for help in the collection of the specimens; Diego Nieto-Lugilde for help with paleotemperature data; Josefa Velasco, Susana Pallarés, José A. Carbonell (Universidad de Murcia) and Rocío Alonso and Pau Balart (IBE, Barcelona) for help with the experiments; and four anonymous reviewers for comments and suggestions. We also thank the Parc del Garraf and his Director Santiago Llacuna for his support and for collecting permits. No permits or ethical approval were required for the experimental procedures. This work was partly funded by project CGL2010-15755 from the Spanish Government to IR.
- Dawson TPS, Jackson T, House JI, Prentice IC, Mace GM. Beyond predictions: biodiversity conservation in a changing climate. Science. 2011;332:53–8.View ArticlePubMedGoogle Scholar
- Sunday JM, Bates AE, Kearney MR, Colwell RK, Dulvy NK, Longino JT, et al. Thermal-safety margins and the necessity of thermoregulatory behavior across latitude and elevation. Proc Natl Acad Sci U S A. 2014;111:5610–5.View ArticlePubMed CentralPubMedGoogle Scholar
- Uvarov BP. Insects and climate. T Roy Ent Soc London. 1931;79:1–232.View ArticleGoogle Scholar
- Bernardo J. Biologically grounded predictions of species resistance and resilience to climate change. Proc Natl Acad Sci U S A. 2014;111:5450–1.View ArticlePubMed CentralPubMedGoogle Scholar
- Culver DC, Pipan T. The biology of caves and other subterranean habitats. Oxford: Oxford University Press; 2009.Google Scholar
- Juan C, Guzik MT, Jaume D, Cooper SJ. Evolution in caves: darwin’s ‘wrecks of ancient life’ in the molecular era. Mol Ecol. 2010;19:3865–80.View ArticlePubMedGoogle Scholar
- Poulson TL, Culver DC. Diversity in terrestrial cave communities. Ecology. 1969;50:153–8.View ArticleGoogle Scholar
- Culver DC. The evolution of aquatic cave communities. Am Nat. 1976;110:945–57.View ArticleGoogle Scholar
- Poulson TL, White WB. The cave environment. Science. 1969;165:971–81.View ArticlePubMedGoogle Scholar
- Janzen DH. Why mountain passes are higher in the tropics? Am Nat. 1967;101:233–49.View ArticleGoogle Scholar
- Futuyma DJ, Moreno G. The evolution of ecological specialization. Annu Rev Ecol Syst. 1988;19:207–33.View ArticleGoogle Scholar
- Huey RB, Kingsolver JG. Evolution of thermal sensitivity of ectotherm performance. Trends Ecol Evol. 1989;4:131–5.View ArticlePubMedGoogle Scholar
- Peck LS, Webb KE, Bailey DM. Extreme sensitivity of biological function to temperature in Antarctic marine species. Funct Ecol. 2004;18:625–30.View ArticleGoogle Scholar
- Latella L, Bernabò P, Lencioni V. Distribution pattern and thermal tolerance in two cave dwelling leptodirinae coleoptera, cholevidae. Subterranean Biol. 2004;6:81–6.Google Scholar
- Culver DC, Sket B. Hotspots of subterranean biodiversity in caves and wells. J Cave Karst Stud. 2000;62:11–7.Google Scholar
- Culver DC, Deharveng L, Bedos A, Lewis JJ, Madden M, Reddell JR, et al. The mid-latitude biodiversity ridge in terrestrial cave fauna. Ecography. 2006;29:120–8.View ArticleGoogle Scholar
- Faille A, Casale A, Balke M, Ribera I. A molecular phylogeny of Alpine subterranean Trechini. BMC Evol Biol. 2013;13:248.View ArticlePubMed CentralPubMedGoogle Scholar
- Faille A, Ribera I, Deharveng L, Bourdeau C, Garnery L, Quéinnec E, et al. A molecular phylogeny shows the single origin of the Pyrenean subterranean Trechini ground beetles (Coleoptera: Carabidae). Mol Phylogenet Evol. 2010;54:97–106.View ArticlePubMedGoogle Scholar
- Ribera I, Fresneda J, Bucur R, Izquierdo A, Vogler AP, Salgado JM, et al. Ancient origin of a Western Mediterranean radiation of subterranean beetles. BMC Evol Biol. 2010;10:29.View ArticlePubMed CentralPubMedGoogle Scholar
- Cieslak A, Fresneda J, Ribera I. Life-history specialization was not an evolutionary dead-end in pyrenean cave beetles. P Roy Soc B Biol Sci. 2014;281:20132978.View ArticleGoogle Scholar
- Rizzo V, Comas J, Fadrique F, Fresneda J, Ribera I. Early pliocene range expansion of a clade of subterranean pyrenean beetles. J Biogeogr. 2013;40:1861–73.Google Scholar
- Hijmans RJ, Cameron SE, Parra JL, Jones PG, Jarvis A. Very high resolution interpolated climate surfaces for global land areas. Int J Climatol. 2005;25:1965–78.View ArticleGoogle Scholar
- Kiehl J, Gent P. The community climate system model, version two. J Climate. 2004;17:3666–82.View ArticleGoogle Scholar
- Otto-Bliesner BL, Brady EC, Tomas R, Levis S, Kothavala Z. Last glacial maximum and holocene climate in CCSM3. J Climate. 2006;19:2526–44.View ArticleGoogle Scholar
- Salt RW. Principles of insect cold-hardiness. Annu Rev Ent. 1961;95:1190–202.Google Scholar
- Lee Jr RE. A primer on insect cold-tolerance. In: Denlinger DL, Lee Jr RE, editors. Low temperature biology of insects. Cambridge: Cambridge University Press; 2010. p. 1–34.Google Scholar
- Calosi P, Bilton DT, Spicer JI, Votier SC, Atfield A. What determines a species’ geographical range? thermal biology and latitudinal range size relationships in European diving beetles (Coleoptera: Dytiscidae). J Anim Ecol. 2010;79:194–204.View ArticlePubMedGoogle Scholar
- Sunday JM, Bates AE, Dulvy NK. Global analysis of thermal tolerance and latitude in ectotherms. P Roy Soc B Biol Sci. 2011;278:1823–30.View ArticleGoogle Scholar
- Hidalgo-Galiana A, Sánchez-Fernández D, Bilton DT, Cieslak A, Ribera I. Thermal niche evolution and geographical range expansion in a species complex of western mediterranean diving beetles. BMC Evol Biol. 2014;14:187.View ArticlePubMed CentralPubMedGoogle Scholar
- Calosi P, Bilton DT, Spicer JI, Atfield A. Thermal tolerance and geographic range size in the Agabus brunneus group of European diving beetles (Coleoptera: Dytiscidae). J Biogeogr. 2008;35:295–305.Google Scholar
- Sánchez-Fernández D, Calosi P, Atfield A, Arribas P, Velasco J, Spicer JI, et al. Reduced salinities compromise the thermal tolerance of hypersaline specialist diving beetles. Physiol Entomol. 2010;35:265–73.View ArticleGoogle Scholar
- Arribas P, Velasco J, Abellán P, Sánchez-Fernández D, Andújar C, Calosi P, et al. Dispersal ability rather than ecological tolerance drives differences in range size between lentic and lotic water beetles (Coleoptera: Hydrophilidae). J Biogeogr. 2012;39:984–94.View ArticleGoogle Scholar
- Salgado JM, Blas M, Fresneda J. Coleoptera: cholevidae. In: Fauna Iberica. Vol. 31. Madrid: Consejo Superior de Investigaciones Científicas; 2008.Google Scholar
- Fresneda J, Hernando C. Speonomus kryophilos n. sp. (Coleoptera, Cholevidae) nuevo Bathysciinae del Pirineo catalán. Ilerda Ciències. 1991;49:259–64.Google Scholar
- Somero GN. Proteins and temperature. Annu Rev Physiol. 1995;57:43–68.View ArticlePubMedGoogle Scholar
- Davis AJ, Jenkinson LS, Lawton JH, Shorrocks B, Wood S. Making mistakes when predicting shifts in species range in response to global warming. Nature. 1998;391:783–6.View ArticlePubMedGoogle Scholar
- Kearney M. Habitat, environment and niche: what are we modelling? Oikos. 2006;115:186–91.View ArticleGoogle Scholar
- Sánchez-Fernández D, Aragón P, Bilton DT, Lobo JM. Assessing the congruence of thermal niche estimations derived from distribution and physiological data. a test using diving beetles. PLoS One. 2012;7:e48163.View ArticlePubMed CentralPubMedGoogle Scholar
- Araújo MB, Ferri-Yáñez F, Bozinovic F, Marquet PA, Valladares F, Chown SL. Heat freezes niche evolution. Ecol Lett. 2013;16:1206–19.View ArticlePubMedGoogle Scholar
- Delay B. Milieu souterrain et écophysiologique de la reproduction et du développement des Coléoptères Bathysciinae hypogés. Mém Biospéol. 1978;5:1–349.Google Scholar
- Frederich M, Pörtner HO. Oxygen limitation of thermal tolerance defined by cardiac and ventilatory performance in spider crab, Maja squinado. Am J Physiol Reg I. 2000;279:R1531–8.Google Scholar
- Pörtner HO. Climate change and temperature-dependent biogeography: oxygen limitation of thermal tolerance in animals. Nature. 2001;88:137–46.Google Scholar
- McCue MD, De Los Santos R. Upper thermal limits of insects are not the result of insufficient oxygen delivery. Physiol Biochem Zool. 2013;86:257–65.View ArticlePubMedGoogle Scholar
- Verberk WCEP, Bilton DT. Respiratory control in aquatic insects dictates their vulnerability to global warming. Biol Lett. 2013;9:20130473.View ArticlePubMed CentralPubMedGoogle Scholar
- Moldovan OT, Jalžić B, Erichsen E. Adaptation of the mouth parts in some subterranean Cholevinae (Coleoptera, Leiodidae). Natura Croatica. 2004;13:1–18.Google Scholar
- Paoletti MG, Beggio M, Dreon AL, Pamio A, Gomiero T, Brilli M, et al. A new foodweb based on microbes in calcitic caves: the Cansiliella (Beetles) case in Northern Italy. Int J Speleol. 2011;40:45–52.View ArticleGoogle Scholar
- Lencioni V, Bernabò P, Latella L. Cold resistance in two species of cave-dwelling beetles coleoptera: cholevidae. J Therm Biol. 2010;35:354–9.View ArticleGoogle Scholar
- Bernabò P, Latella L, Jousson O, Lencioni V. Cold stenothermal cave-dwelling beetles do have an HSP70 heat shock response. J Therm Biol. 2011;36:206–8.View ArticleGoogle Scholar
- Mermillon-Blondi F, Lefour C, Lalouette L, Renault D, Malard F, Simon L, et al. Thermal tolerance breadths among groundwater crustaceans living in a thermally constant environment. J Exp Biol. 2013;216:1683–94.View ArticleGoogle Scholar
- Eme D, Malard F, Colson-Proch C, Jean P, Calvignac S, Konecny-Dupré L, et al. Integrating phylogeography, physiology and habitat modelling to explore species range determinants. J Biogeogr. 2014;41:687–99.View ArticleGoogle Scholar
- Zachos J, Pagani M, Sloan L, Thomas E, Billups K. Trends, rhythms, and aberrations in global climate 65 Ma to present. Science. 2001;292:686–93.View ArticlePubMedGoogle Scholar
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.