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Fig. 5 | BMC Evolutionary Biology

Fig. 5

From: Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies

Fig. 5

Genomic in situ hybridization (GISH) in pachytene oocytes of Leptidea juvernica (a–d), L. sinapis (e–h) and L. reali (i–l). Female-derived genomic probes were labelled with fluorescein-12-dUTP (green) and chromosomes were counterstained with DAPI (blue). Figures (ad), (eh) and (il) show detailed analyses of sex chromosome multivalents W1-nZ1-n: (a, e, i) merged images of female genomic probes and DAPI staining; (b, f, j) DAPI images; arrows indicate DAPI-positive W-chromosome segments and heterochromatic blocks at the end of the W chromosomes; (c, g, k) hybridization pattern of the female genomic probes; the asterisk indicates an undifferentiated segment of one of the W chromosomes; (d, h, l) schematic drawings of the sex chromosome multivalents. Scale bar = 10 μm

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