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Fig. 6 | BMC Evolutionary Biology

Fig. 6

From: Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies

Fig. 6

Comparative genomic hybridization (CGH) in pachytene oocytes of Leptidea juvernica (a–e), L. sinapis (f–j) and L. reali (k–o). Female-derived genomic probes were labelled with fluorescein-12-dUTP (green), male-derived genomic probes were labelled with Cy3-dUTP (red) and chromosomes were counterstained with DAPI (blue). Figures (ae), (fj) and (ko) show detailed analyses of sex chromosome multivalents W1-nZ1-n: (a, f, k) merged images of both genomic probes and DAPI staining; (b, g, l) DAPI images; (c, h, m) female genomic probes; arrows indicate W-chromosome segments with female-specific hybridization pattern; (d, i, n) male genomic probes; (e, j, o) schematic drawings of the sex chromosome multivalents. Scale bars = 10 μm

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