Fig. 6
From: Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies
Comparative genomic hybridization (CGH) in pachytene oocytes of Leptidea juvernica (a–e), L. sinapis (f–j) and L. reali (k–o). Female-derived genomic probes were labelled with fluorescein-12-dUTP (green), male-derived genomic probes were labelled with Cy3-dUTP (red) and chromosomes were counterstained with DAPI (blue). Figures (a–e), (f–j) and (k–o) show detailed analyses of sex chromosome multivalents W1-nZ1-n: (a, f, k) merged images of both genomic probes and DAPI staining; (b, g, l) DAPI images; (c, h, m) female genomic probes; arrows indicate W-chromosome segments with female-specific hybridization pattern; (d, i, n) male genomic probes; (e, j, o) schematic drawings of the sex chromosome multivalents. Scale bars = 10 μm