Fig. 3From: Insights into the evolution of enzyme substrate promiscuity after the discovery of (βα)8 isomerase evolutionary intermediates from a diverse metagenomePhylogenetic analysis of HisA / PriA evolutionary intermediates. A Bayesian phylogenic tree of HisA homologs is shown. Minimum branch support is 0.7. HisA, PriA and intermediate sequences, as CAM1, are grouped in different clades, labeled in red, blue and green, respectively. The key enzymes HisA_Afer (C7LZ82) and PriA_Blon (Q8G4S5) are also shown. The evolutionary intermediate, reflected at the codon level in the N-PBS motif, is shown. Functionally analyzed proteins, and their first N-PBS amino acid codon usage, are marked with an asteriskBack to article page