Enzymes | In vivo activity | In vitro activitya | ||||||
---|---|---|---|---|---|---|---|---|
ProFAR isomerase (HisA) | PRA isomerase (TrpF) | |||||||
HisA | TrpF | K M μM | k cat s−1 | k cat/K M s−1 μM−1 | K M μM | k cat s−1 | k cat /K M s−1 μM−1 | |
HisA_Afer | + | - | 1.1 ± 0.2 | 0.05 ± 0.001 | 0.045 | n.d. | n.d. | n.d. |
PriA_Blon | + | + | 2.7 ± 0.5 | 0.4 ± 0.1 | 0.1 | 6.1 ± 0.1 | 2.1 ± 0.5 | 0.3 |
CAM1 | + | + | 1.7 ± 0.1 | 0.3 ± 0.03 | 0.2 | 40 ± 7 | 3.5 ± 0.04 | 0.09 |
CAM1_A81G | + | + | 1.7 ± 0.2 | 0.1 ± 0.01 | 0.06 | 32.2 ± 1.7 | 1.9 ± 0.1 | 0.06 |
CAM1_A81S | + | + | 4.0 ± 0.9 | 0.2 ± 0.03 | 0.04 | 23.5 ± 6.5 | 0.5 ± 0.1 | 0.02 |
CAM2 | + | - | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |