Morphological and ecological divergence of Lilium and Nomocharis within the Hengduan Mountains and Qinghai-Tibetan Plateau may result from habitat specialization and hybridization

Table 3 Primers and sequences statistics of nuclear and chloroplast makers used in present study

Region	Forward-primer (5′-3′)	Reverse-primer (5′-3′)	Reference	Alignment length (bp)	Variable sites	Parsimony informative sites
ITS	GGAAGTAAAAGTCGTAACAAGG	TCCTCCGCTTATTGATATGC	[92]	673	398	287
rbcL	ATGTCACCACAAACAGAGAC	TCACATGTACCCGCAGTAGC	[93]	796	84	42
matK	CGATCTATTCATTCAATATTTC	TCTAGCACACGAAAGTCGAAGT	[94]	392	33	23
trnL intron and trnL-trnF spacer	CGAAATCGGTAGACGCTACG	ATTTGAACTGGTGACACGAG	[95]	786	57	34
rpl32-trnL(UAG)	CAGTTCCAAAAAAACGTACTTC	CTGCTTCCTAAGAGCAGCGT	[45]	842	138	100
psbA-trnH	ACTGCCTTGATCCACTTGGC	CGAAGCTCCATCTACAAATGG	[96]	613	24	19
			Total plastid	3429	336	218

ISSN: 2730-7182