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Table 1 Positively selected sites in octopus haemocyanin

From: Positive selection in octopus haemocyanin indicates functional links to temperature adaptation

Residue

SLAC

FEL

MEME

FUBAR

EF

PRIMEa

TreeSAAPb

pHi (8)

pK’ (8)

pα (6)

PCT (6)

2383

 

*

*

       

↓(1)

  
 

*

         

↑(2–4)

  

2409

             

2410

          

↓(5–8)

  

2442

     

(CC)

    

↓(10) ↑(5,9,11)

  

2469

     

CC

     

↓(12,13)

 

2496

         

↓(14,15)

   

2503

     

(P)

     

↓(14,15,22-27) ↑(20)

 

2545

          

↓(4, 20, 28–30) ↑(9,19,31,32)

  

2575

           

↓(33,34)

 

2585

     

pHi

CC

(P)

(V)

    

2602

         

↓(35,36)

  

↑(35,36)

2610

     

pHi

   

↓(37–39) ↑(40,41)

  

↓(40–42) ↑(37–39)

2643

     

CC

      

↓(38)

  1. Sites at which at least three or more selection tests identified significant positive selection. Analysis was performed for two separate alignments, containing 113 or 126 sequences respectively, and covering in total a 396 amino acid long region of the haemocyanin’s functional units f and g. Numbering of positions refers to the published full haemocyanin sequence of Enteroctopus dofleini [UniProt: O61363] [23, 24]. Significance thresholds were: P ≤ 0.10 for SLAC, ≤ 0.10 for FEL, MEME and PRIME; Posterior Probability ≥ 0.90 for FUBAR; Bayes Factor ≥ 0.50 for EF. See Additional file 5: Table S5 for detailed results.
  2. Significant test result ; CC Selection for this property; (CC) Conservation of this property
  3. aCC Chemical Composition; P Polarity; V Volume; pHi Iso-electric point; H Hydropathy. b pHi isoelectric point; pK’ equilibrium constant (ionization of COOH); pα alpha helical tendencies; PCT Power to be at the C-terminal (Magnitude of amino acid change)