Fig. 1
From: ERK1 and ERK2 present functional redundancy in tetrapods despite higher evolution rate of ERK1
ERK1 and ERK2 protein appear first in bony vertebrates (Bichir). Western-blot analysis of samples from brain, spinal cord or cultured cells: immunoblots were incubated with polyclonal anti-ERK antibody (ERK1 and ERK2; panels a, b, c) or anti-phosphoERK antibody (pERK1 and pERK2; panels d, e, f). Taxonomic names are listed in Additional file 7. Predicted MW sizes of vertebrate ERKs range from 41.7 kDa for lamprey’s ERK to 44.1 for zebrafish’s ERK1. SC: spinal cord; CE: cerebellum; OP: optic lobe. Positions of ERK1, ERK2, phospho-ERK1 (pERK1) and phospho-ERK2 (pERK2) are indicated on the sides