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Fig. 3 | BMC Evolutionary Biology

Fig. 3

From: Detailed reconstruction of the nervous and muscular system of Lobatocerebridae with an evaluation of its annelid affinity

Fig. 3

General and detailed organization of the nervous system of Lobatocerebrum riegeri n. sp. as seen with CLSM. DAPI in cyan, FMRF in purple and acetylated α-tubulin in yellow or “glow”. All images are maximum intensity projections of a subset of the original image stack on various locations of the body if not specified below a Dorsal view of a juvenile specimen, b Lateral view of an adult, pharyngeal ganglia outlines in red, details of the same specimen as (c), c Three different substacks of an adult specimen pieced together for an overview-picture. The specimen is twisted and some portions are laterally oriented and others dorso-ventrally oriented , d Dorsal view of the details of the brain, e Juvenile showing the general organization of the nervous system. Notice the presence of only three trunk commissures, f Dorsal view of the details of the brain in a juvenile. Details of the same specimen as (e), g Dorsal view of details of the origin of the median nerve cord in a juvenile. Details of the same specimen as (e). Abbreviations: avnc: anterior ventral nerve cord, br: brain, c1–4: commissure 1 - 4, dcn: dorso-anterior commissure of the neuropil, fg: frontal gland, hg: hindgut, lc: caudal lobe, lca: major caudal lobe, lci: minor caudal lobe, lr: rostral lobe, mnc: median nerve cord, mo: mouth opening, nlca: nerve of the major caudal lobe, nlr: nerve of the rostral lobe, np: neuropil, pfg: posterior frontal glands, pg1–2: postpharyngeal ganglia 1 - 2, pp: posterior projection, spd: spermioduct, t: testes, tc: terminal commissure, vlnc: posterior ventro lateral nerve cord

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