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Fig. 5 | BMC Evolutionary Biology

Fig. 5

From: Karyotype differentiation in 19 species of river loach fishes (Nemacheilidae, Teleostei): extensive variability associated with rDNA and heterochromatin distribution and its phylogenetic and ecological interpretation

Fig. 5

Karyotypes of male and female of S. fasciolata after different cytogenetic protocols. a female (2n = 50), b-d male (2n = 51). a, c conventional Giemsa staining, b dual-colour FISH with 45S rDNA (red, arrows) and 5S rDNA (green, arrowheads) probes, d C-banding. Putative sex chromosomes of female are boxed b, d next to those of the male karyotype. Note the presence of 5S rDNA site (b) and prominent pericentromeric heterochromatic region (d) on a putative X chromosome. Notice also a centromeric C-band on Y1 (d). Bar = 10 μm

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