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Fig. 5 | BMC Evolutionary Biology

Fig. 5

From: Evaluation of the mechanisms of intron loss and gain in the social amoebae Dictyostelium

Fig. 5

High GC content surrounding discordant intron positions. The difference in the distribution of GC or relative GC content between the two compared items could be perceived by comparing each percentile. a Exonic sequences flanking lost introns have significantly higher GC contents than the exonic sequences flanking conserved introns of the same species. b Exonic sequences flanking the extant introns at discordant intron positions have significantly higher GC contents than the conserved introns of the same species. c At discordant intron positions, exonic sequences flanking lost introns have significantly higher relative GC contents than the exonic sequences flanking extant introns of the other species. The relative GC content surrounding a discordant intron position was defined as the ratio of the GC content surrounding the position divided by the median value of the GC content surrounding the conserved introns of the same gene. A total of 407 lost intron positions in Dictyostelium discoideum, 178 lost intron positions in Dictyostelium purpureum, and 5,724 conserved intron positions were surveyed. The numbers of intron-lost genes surveyed were 201 and 109 for Dictyostelium discoideum and Dictyostelium purpureum, respectively. As the data are not normally distributed, we used nonparametric analyses in our comparisons of the data

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