Fig. 4

Numbers of intrinsically disordered proteins (IDPs) versus numbers of N/Q-rich proteins or prion predictions. a Plot of number of IDPs versus numbers of N/Q-rich proteins. Proteins with ID regions >30 residues were counted as IDPs. We only consider IDPs that do not have N/Q-rich domains in the IDP totals. Saccharomycetes species are red points, and non-Saccharomycetes blue. The trend line for both is shown. The Pearson correlation coefficients are: R = 0.135 (P = 0.03) Saccharomycetes, R = 0.358 (P < 1e–07) non-Saccharomycetes. b Same as (A), but with IDPs versus predicted prion proteins (the union of PAPA and PLAAC predictions for each proteome) that are N/Q-rich. R = 0.139 (P = 0.03) Saccharomycetes, R = 0.444 (P < 1e–07) non-Saccharomycetes. The IDP totals are for those that have no prion predictions in them (by either PAPA or PLAAC), i.e. all of the proteins with prion predictions are removed. c Same as (A), but for the subset of predicted prions that are not N/Q-rich. R = 0.49 (P < 1e–07) Saccharomycetes, R = 0.422 (P < 1e–07) non-Saccharomycetes. To define non-N/Q-rich prion predictions, we use a strict threshold for N/Q bias (P = 1×10⁻⁵). As above in part (B), the IDP totals are for those that have no prion predictions in them