Fig. 2

Homophilic adhesion of PCDHB11 and PCDHGA3 in human suspension cells. GFP was electroporated alone (a) or in combination with human PCDHB11 (b) or murine PCDHGA3 (c) into K562 cells; in the same way, mCherry was electroporated alone (d) or in combination with reverted PCDHB11 (e) or murine PCDHGA3 (f). Scale bar: 250 μm. g Fraction of large clusters (>30 cells), medium clusters (10–30 cells) or small clusters (<10 cells) in each condition. h Adhesive strength of cells in each condition as proportion of clusters with ≥ 10 cells, relative to the condition with the same fluorescent protein and PCDHGA3. Error bars: standard error of mean. The three levels of protocadherin (none, PCDHB11, PCDHGA3) were significantly different from each other (ANOVA, p = 0.0006; Tukey HSD test: none vs. PCDHB11, p = 0.036; none vs. PCDHGA3, p = 0.0005; PCDHB11 vs. PCDHGA3, p = 0.026; n = 4 transfections)