Fig. 4
From: Functional test of PCDHB11, the most human-specific neuronal surface protein
Specificity of adhesion of PCDHB11. Different populations of K562 cells were separately electroporated as in Fig. 2, where one population received GFP and a protocadherin, and the other mCherry and a protocadherin. Green and red populations were mixed 24-48 h later, rocked for 2 h and then photographed while still alive. a-p The identity of the protocadherins in the green and red populations are indicated. Scale bar: 250 μm. q For each pair of cell populations, the conditions with switched fluorophores were joined, and the proportion of mixed clusters (containing both red and green cells) was expressed as fraction of all clusters. In an overall 2-way ANOVA, the identity of the cell populations and their interaction were each highly significant (n = 17-84 images per condition, p < 10- 8). In each subpanel, the identity of the second cell population was significant (ANOVA, p < 0.05). In each subpanel, the second cell populations were significantly different from control where indicated (linear models with Bonferroni correction for comparison with control; *:p < 0,05; **: p < 0.01; ***: p < 0.001)