Fig. 1

Expression analysis of regulatory and terminal differentiation orthologs of known pancreatic genes. mRNA localization of sea urchin regulatory (a-f, green) and terminal differentiation (h-m, magenta) genes. For all the figures in this paper, every picture is a full projection of merged confocal Z stacks and nuclei are stained with DAPI and depicted in blue. Red circles in f show Mist1 RNA localization in alternating cells of the apical organ. Inset in panel k is a representative single confocal section of the upper stomach of a late larva showing that SpCpa2L transcripts are abundantly expressed in the entire cell. g, n Temporal expression profiles of pancreatic regulatory and terminal differentiation genes during sea urchin development. The graphs show the relative transcript abundance normalized against ubiquitin mRNA. The results are expressed as percentage of the maximum value, corresponding to the stage with the highest level of expression. Standard deviations of three technical replicas are all <0.5. For the sake of simplicity, for each panel, the species in the gene name has been omitted. Abbreviations: d, days; dv, dorsal view; h, hours post fertilization; vv, ventral view