Fig. 4

Analysis of gene expression after MO perturbation. Ptf1a mRNA localization in control embryos (a) and in embryos injected with MO directed against the translation of SpHnf1 RNA (b). Cpa2L, Pnlp2/5, Amy3, Ptf1a and Fng transcripts were detected by single or double FISH in control larvae (c, e, g, i) and in larvae injected with two different MOs directed against the translation of SpPtf1a RNA (d, f, h, j). Note that f, h and j show larvae injected with SpPtf1a MO1. To confirm the effects of MO1, figure d shows that SpCpa2L transcripts are absent also in larvae injected with SpPtf1a MO2. Inset in j is a representative single confocal section of the dorsal upper stomach of a late larva and the white arrow shows that Ptf1a + and Fng + cells are adjacent and not overlapping. k qPCR analysis showing the effects of SpPtf1a MO1 perturbation on transcript levels of selected pancreatic genes at 70 h. Fold changes ≥ -2 and ≤ 2 are shaded in light grey and indicate non-significant changes in gene expression