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Fig. 5 | BMC Evolutionary Biology

Fig. 5

From: A pancreatic exocrine-like cell regulatory circuit operating in the upper stomach of the sea urchin Strongylocentrotus purpuratus larva

Fig. 5

Sea urchin Ptf1a is active and can function together with mammalian Ptf1 partners. a 293 t cells were transiently transfected with luciferase reporter containing 4x Ptf1a-responsive element (vector indicated as 4x in the figure) and/or spPtf1a, E47, Rbpl construct; renilla vector was cotransfected for normalization. Results are displayed in the box plot as fold activation over the activity of 4x reporter alone. Transfections were done in triplicate and were repeated 5 times. Luciferase assays from all transfections show similar trend, one representative result is shown. t-test was carried out for group comparison. The bottom and top of the box are the first and third quartiles, and the band inside the box is the median. The whisker represents 1.5 IQR (interquantile range, the differences between the third and first quantile). b SpPtf1a has lower activity compared with mammalian Ptf1a in the in vitro luciferase assay. Experiment setting is the same as (a) except that rat rnPtf1a was transfected in a second plate side-by-side for comparison. We tested SpPtf1a activity in multiple cell lines, one representative result from HeLa cells is shown. c Alignment of the closest to the transcription start site (TSS) Ptf1a binding sites found by bioinformatic analysis on S. purpuratus (Sp) promoters with the canonical Ptf1a binding site of the RnCtbr1 gene (ref). nt, nucleotides

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