Experimental evolution under hyper-promiscuity in Drosophila melanogaster

Table 6 Sperm precedence

Phenotype	Test	Generation	Mating number	SPR- proportion sired ± S.E.	Control mean proportion sired ± S.E.	t-value	df	P
P1^a	Effects of SPR- in unselected males	N/A	1^st	0.06 ± 0.04	0.15 ± 0.03	3.3	1,36	0.08
			5^th	0.04 ± 0.01	0.05 ± 0.01	0.1	1,36	0.82
	Effects of SPR- in experimentallly-evolved backcrossed controls	N/A	1^st	0.17 ± 0.01	0.12 ± 0.01	−1.0	1,284	0.31
			5^th	0.07 ± 0.01	0.11 ± 0.01	−0.7	1,224	0.47
	Experimental evolution of males	36	1^st	0.14 ± 0.02	0.18 ± 0.02	−1.4	1,6	0.20
			5^th	0.06 ± 0.01	0.08 ± 0.01	−1.5	1,6	0.19
P2^b	Effects of SPR- in unselected males^b	N/A	1^st	0.88 ± 0.03	0.89 ± 0.04	0.2	1,35	0.60
	Effects of SPR- in experimentally-evolved backcrossed controls	N/A	1^st	0.78 ± 0.12	0.91 ± 0.06	2.5	1,221	0.01
	Experimental evolution of males	36	1^st	0.85 ± 0.02	0.81 ± 0.02	2.2	1,6	0.07

^aFull model results in Additional file 1: Table S6
^bFull model results in Additional file 1: Table S7
Effects of the SPR- deficiency itself, or experimental evolution in SPR- and control populations, on the proportion of offspring sired by a focal male as the first or second male to mate with a wild-type female (P1 or P2, respectively). P1 tests include a male’s first and fifth mating to test for differences in male ejaculate depletion across successive matings. Effects of the SPR deficiency itself were evaluated in unselected flies or in experimentally evolved backcrossed or outcrossed controls (see Methods)

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