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Fig. 6 | BMC Evolutionary Biology

Fig. 6

From: Evolution of RLSB, a nuclear-encoded S1 domain RNA binding protein associated with post-transcriptional regulation of plastid-encoded rbcL mRNA in vascular plants

Fig. 6

Levels of RLSB-a and RLSB-b transcript accumulation across maize leaf developmental gradients in wild type and rlsb-a1/rlsb-a2 insertion mutant plants. The transcription levels of the two maize RLSB paralogs RLSB-a and RLSB-b across wild type and mutant gradients maize leaf gradients were analyzed by qRT-PCR using primers highly specific for each sequence, as described in Methods. Sections used for sampling along the leaf gradients are indicated by dashed lines. Panel a Relative levels of mRNA accumulation for RLSB-a and RLSB-b transcripts in wild type RLSB/RLSB maize seedlings. Panel b Relative levels of mRNA accumulation for RLSB-a and RLSB-b transcripts in rlsb-a1/rlsb-a2 mutant maize seedlings. Quantification of transcript levels in both cases was standardized to actin mRNA. Data was averaged for three wild type and three mutant siblings, with three technical repeats for each of the plant samples. Statistical significance was calculated using Student’s t-test, as described in materials and methods. Note that the expression levels of RLSB-a and RLSB-b from the seven sections of the mutant plants show very little correlation (r 2 = 0.335), suggesting a markedly different trend in the expression patterns of these two homologs in the mutant plants. For each bar, P values were less than 0.05

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