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Table 2 Pharmacological characterization of PPAR agonist and antagonist regulation of spotted gar fabp1 promoter activity

From: Subfunctionalization of peroxisome proliferator response elements accounts for retention of duplicated fabp1 genes in zebrafish

  WY14643
  Upward Slope Downward Slope
  - GW6471 T0070907 - GW6471 T0070907
E max (RLU) 80.6 ± 7.40 97.5 ± 3.78 74.2 ± 9.63 - - -
E min (RLU) 10.9 ± 4.64 8.86 ± 3.50 8.93 ± 4.72 24.7 ± 4.88** - 21.6 ± 4.13**
EC50 (μM) 0.12 (0.07–0.28) 0.54 (0.43–0.65)* 0.17 (0.05–0.25) 0.69 (0.52–1.1)** 0.45 (0.22–0.71)**
  Rosiglitazone
  Upward Slope Downward Slope
  - GW6471 T0070907 - GW6471 T0070907
E max (RLU) 56.3 ± 2.40 57.4 ± 2.44 42.5 ± 4.50 - - -
E min (RLU) 5.28 ± 4.80 −0.42 ± 2.73 1.21 ± 7.80 - - -
EC50 (μM) 0.009 (0.006–0.017) 0.016 (0.012–0.021) 0.024 (0.021–0.062)* - - -
  1. Data derived from Fig. 6 presented as the mean ± SEM or 95 % confidence intervals (brackets)
  2. *P < 0.05 compared to agonist treatment alone within the upward slope
  3. **P < 0.05 compared to matched treatment between upward and downward slopes, as determined via two-way ANOVA followed by Bonferroni’s post-hoc analysis for E max, E min, or by non-overlapping confidence intervals (EC50). n = 34