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Table 2 Pharmacological characterization of PPAR agonist and antagonist regulation of spotted gar fabp1 promoter activity

From: Subfunctionalization of peroxisome proliferator response elements accounts for retention of duplicated fabp1 genes in zebrafish

 

WY14643

 

Upward Slope

Downward Slope

 

-

GW6471

T0070907

-

GW6471

T0070907

E max (RLU)

80.6 ± 7.40

97.5 ± 3.78

74.2 ± 9.63

-

-

-

E min (RLU)

10.9 ± 4.64

8.86 ± 3.50

8.93 ± 4.72

24.7 ± 4.88**

-

21.6 ± 4.13**

EC50 (μM)

0.12 (0.07–0.28)

0.54 (0.43–0.65)*

0.17 (0.05–0.25)

0.69 (0.52–1.1)**

0.45 (0.22–0.71)**

 

Rosiglitazone

 

Upward Slope

Downward Slope

 

-

GW6471

T0070907

-

GW6471

T0070907

E max (RLU)

56.3 ± 2.40

57.4 ± 2.44

42.5 ± 4.50

-

-

-

E min (RLU)

5.28 ± 4.80

−0.42 ± 2.73

1.21 ± 7.80

-

-

-

EC50 (μM)

0.009 (0.006–0.017)

0.016 (0.012–0.021)

0.024 (0.021–0.062)*

-

-

-

  1. Data derived from Fig. 6 presented as the mean ± SEM or 95 % confidence intervals (brackets)
  2. *P < 0.05 compared to agonist treatment alone within the upward slope
  3. **P < 0.05 compared to matched treatment between upward and downward slopes, as determined via two-way ANOVA followed by Bonferroni’s post-hoc analysis for E max, E min, or by non-overlapping confidence intervals (EC50). n = 34