Fig. 2

Multiple sequence alignment of FPLs. Multiple sequence alignment of selected fluorescent protein orthologs (FPLs) from ctenophores and fluorescent proteins. Sequences are as follows: M. leidyi ML181711a is the manually corrected protein from the M. leidyi genome; H. beehleri FPL is the ctenophore FPL from the H. beehleri transcriptome in this study; A. victoria GFP is the canonical GFP sequence; Hbee FP 2010 is the FP sequence described by Haddock et al. (2010); H. beehleri transcriptome FP was identified from the same H. beehleri transcriptome for this study. Intensity of blue color indicates conservation at the position. Residues involved in chromophore formation in FPs are shown in green, while the unconserved residues at the same positions in ctenophore FPLs are shown in red. The conserved N-terminal motif in the ctenophore FPLs is shown in gray, where unconserved residues are highlighted in pink. Amino acid differences between the two H. beehleri FPs are shown in yellow; there are only four differing positions, suggesting the two FPs are alleles