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Fig. 4 | BMC Evolutionary Biology

Fig. 4

From: The evolutionally-conserved function of group B1 Sox family members confers the unique role of Sox2 in mouse ES cells

Fig. 4

Mouse ES cells maintained by Drosophila SoxN retain pluripotency. a A strategy to eatablish mouse ES cells maintained by Drosophila SoxN. 2CG2 ES cells were engineered according to the steps shown. The resulting ES cells, designated Hae ES cells, express Drosophila SoxN under the regulation of the endogenous Sox2 promoter. b Immunostaining of Hae ES cells. In the column of αSox2, D and M indicate the staining with anti-SoxN antisera and mouse anti-Sox2 monoclonal antibody, respectively. Hae ES cells express Drosophila SoxN but not mouse Sox2. Nuclear signals indicate the antibody staining whereas the cytoplasmic fluorescence signals were derived from DsRed. c Chimeric embryos generated by injection of Hae ES cells expressing DsRed (right side of each panel) with the negative control (left side)

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