Molecular phylogeny and timing of diversification in Alpine Rhithrogena (Ephemeroptera: Heptageniidae)

Table 1 Sequence variation for each gene region and for the three concatenated matrices (mitochondrial: cox1 + 16S; nuclear: PEPCK + EF-1α + wg; combined: all five gene fragments combined)

Data set (n = 38)	bp	K	S	S _i	%S _i	Model
cox1	658	38	237	217	33	GTR + Γ + I
16S	511	36	67	45	9	GTR + Γ + I
PEPCK	557	37	154	94	17	HKY + Γ
EF-1α	139	24	27	12	9	K2P + Γ
wg	463	27	31	11	2	GTR + I
mitochondrial	1169	38	304	262	22
nuclear	1147	37	212	120	10
combined	2316	38	516	383	17

n = number of sequences, bp = size of aligned matrices, K = number of haplotypes (cox1, 16S) or genotypes (PEPCK, EF-1α, wg) or a combination of both (combined), S = number of polymorphic sites, S _i = number of parsimony-informative sites, Model = best evolutionary model selected following the second-order Akaike information criterion (AICc). Concatenated matrices were examined with partitioned analysis using the best model for each gene fragment (see Methods)

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