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Corepressor diversification by alternative mRNA splicing is species specific
© The Author(s). 2016
- Received: 20 April 2016
- Accepted: 30 September 2016
- Published: 19 October 2016
SMRT and NCoR are corepressor paralogs that help mediate transcriptional repression by a variety of transcription factors, including the nuclear hormone receptors. The functions of both corepressors are extensively diversified in mice by alternative mRNA splicing, generating a series of protein variants that differ in different tissues and that exert different, even diametrically opposite, biochemical and biological effects from one another.
We report here that the alternative splicing previously reported for SMRT appears to be a relatively recent evolutionary phenomenon, with only one of these previously identified sites utilized in a teleost fish and a limited additional number of the additional known sites utilized in a bird, reptile, and marsupial. In contrast, extensive SMRT alternative splicing at these sites was detected among the placental mammals. The alternative splicing of NCoR previously identified in mice (and shown to regulate lipid and carbohydrate metabolism) is likely to have arisen separately and after that of SMRT, and includes an example of convergent evolution.
We propose that the functions of both SMRT and NCoR have been diversified by alternative splicing during evolution to allow customization for different purposes in different tissues and different species.
- mRNA splicing
The nuclear hormone receptors are ligand-modulated transcription factors involved in the regulation of many developmental and homeostatic processes (1–8). The transcriptional properties of these receptors are mediated through their recruitment and release of auxiliary proteins, denoted corepressors and coactivators [1–8]. SMRT and NCoR are important corepressors for the nuclear hormone receptors and for many additional, non-receptor transcription factors [3, 9–15]. Both SMRT and NCoR function as bridging proteins, possessing both specific domains that recruit them to their transcription factor partners and separate regions that bind additional components of a larger corepressor complex; the complex inhibits transcription by modifying the chromatin template and/or by interfering with components of the transcriptional machinery [3, 16].
SMRT and NCoR are present as two distinct loci in vertebrate genomes. In contrast, only single SMRT/NCoR-like loci have been detected in the genomes of other deuterostomes, including the non-vertebrate chordata and echinoderms . This supports the concept that NCoR and SMRT are paralogs that arose from an ancestral gene duplication and divergence. More weakly related loci (e.g., SMRTER and GEI-8) are found in yet more divergent phyla such as the arthropoda and nematoda [18, 19] although these bear only isolated blocks of sequence relatedness and lack many other structural features found in the chordate NCoR/SMRT (such as the “CoRNR box” motifs/Receptor Interaction Domains (RIDs) responsible for the interaction of the vertebrate corepressors with their nuclear receptor partners).
Isolation of RNA from tissues and organs
All animals were maintained and euthanized using University of California at Davis authorized protocols and with the written approval of the University of California at Davis Animal Use committee; samples of human materials were previously collected and encoded to ensure anonymity, allowing them to be defined as exempt from regulation by the U. C. Davis Internal Review Board. Livers, kidneys, skeletal muscle, whole brain, cerebral cortex, cerebellum, brain stem, S1 cortex, hippocampus, and magnocellular preoptic nuclei were obtained by manual dissection. Biological samples were typically stored in RNAlater solution (Life Technologies, Grand Island, NY) at −20 or −80 °C before RNA extraction. Total RNA was isolated from approximately 35 mgs wet weight of each organ using a RNAeasy mini-kit (Qiagen, Hilden, Germany) and manufacturer’s directions [24, 29].
cDNA synthesis and reverse transcriptase PCR quantitation
Approximately 1 μg of total RNA from each sample was converted into cDNA using a QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany) employing the manufacturer’s directions and including a DNA-wipeout pre-step to avoid genomic contamination [24, 29]. The products were then typically diluted with RNase-free water to 120 μl, with 2 μl of which used per PCR reaction. The PCR reactions were performed in a total of 20 μl volume per reaction using the GoTaq enzyme, buffers (Promega, Madison WI), and splice-specific primer pairs designed to flank each known alternative splice-site. The multiple alternatively spliced locations within a corepressor mRNA were therefore analyzed by using a panel of primer pairs, each specific for an alternatively spliced location [24, 26, 29] (Additional file 1: Table S1). PCR was performed at 94 °C for 2 mins., followed by 30 cycles of 30 s. at 94 °C, 30 s. at 53 °C, 60 s. at 72 °C, and a final 5 min. extension at 72 °C prior to temporary storage at −20 °C.
The PCR products for each primer pair were then resolved by electrophoresis in a 2 % agarose gel in 1X TAE buffer, and the DNA was stained with ethidium bromide and was quantified by use of a digital camera and AlphaEase software (version 3.1.2) [24, 26, 29]. The abundance of each alternatively spliced corepressor isoform was calculated as a percentage of the sum of all the alternatively spliced isoforms produced at that alternative splice location (the primers were designed such that each isoform yielded a distinct-sized PCR product) [24, 26, 29]. Although this method does not quantitate absolute levels of each RNA, it does permit the relative ratio of the splice variants produced at any given alternative splice site to be accurately measured: the technique is internally controlled for primer/PCR reaction efficiency, is insensitive to possible non-uniformity in lane loading, electrophoresis, staining, or destaining, and its reproducibility has been confirmed in practice by the low standard deviations observed when this approach was repeated at distinct times or by different researchers [24, 26, 29]. Quantitative RT-PCR could not be used in this context because many corepressor variants differ from others only due to lack of specific exons, prohibiting the design of oligonucleotide primer pairs unique to these variants [24, 26, 29].
Each sample was analyzed by PCR a minimum of 3 times and the average and standard error calculated. For Danio, Gallus, Mus, and Homo (representing a wide range of taxonomy) we were able to analyze samples from three or more individuals from each species; the variation between these individuals was small and essentially equal to the variation when a sample from one individual was analyzed multiple times (the corresponding figures include this data from multiple individuals). It was more difficult to obtain multiple individuals of Xenopus, Monodelphis, or Trachemys due to practical or ethical reasons; here too, however, there were only minimal variations when different individuals at distinct developmental stages or different aliquots of the same tissue were analyzed individually (please see Results). We also emphasize that our conclusions are based on the qualitative presence or absence of a given splice form, rather than on any modest quantitative differences in splice abundance.
A limited form of the known alternative splicing of SMRT, but not of NCoR, could be detected early in phylogeny in the teleost zebrafish (Danio rerio). We began with liver as a model organ; the livers of mice and Xenopus express a representation of all the alternative spliced variants of both SMRT and NCoR reported for these species . Notably our analysis was designed to detect all of the known splice sites that had been previously identified in Xenopus, mice, or humans .
A comparable analysis of a bird, (Gallus gallus), displayed a mix of the features found in Danio and Trachemys (Fig. 4c and Additional file 1: Figure S1D). Notably we see very little or none of the SMRT exon 40b + splice variant in the liver, but we do detect significant expression of the SMRT exon 47b+/b−/− alternatively spliced variants. Again no confirmed alternative splicing was observed at any of the other SMRT or any NCoR positions tested.
Essentially comparable alternative splicing of SMRT and NCoR was observed at two earlier Xenopus developmental stages, although some variants were expressed at slightly different relative abundances at the different stages (Fig. 5b and c).
We next examined these alternative splicing events in different regions of mouse brain: S1 cortex, hippocampus, and magnocellular preoptic nucleus (a subregion of the mouse thalamus) (Fig. 7). The locations of the alternative splicing sites in mouse brain paralleled those observed in mouse liver (Fig. 7), although we did see the enrichment of the SMRT exon 40b + and NCoR exon 37b- splice variants in these subregions as previously noted for whole brains. Very similar results were also obtained with mouse brain dorsal thalamus and hypothalamus (Additional file 1: Figure S2C and data not shown).
Molecular basis of taxonomic variation in alternative corepressor splicing. What was the molecular basis behind the differences of alternative corepressor splicing observed among the species examined? In several species the absence of detectable splice forms was consistent with the absence of appropriate cis-acting consensus splice donor sequences from the corresponding splice sites (such as the absence of an appropriately located GT donor sequence that would be required to generate a Danio NCoR exon 37b + splice variant ). In many other species, however, the necessary splice donor or acceptor sequences are present  yet alternative splicing originating from these sites was not observed in practice. Presumably a lack of appropriate trans-acting factors in these species, or alterations of yet-unidentified cis-sequences within the relevant introns, account for this absence of alternative splicing at these locations. Differences in trans-acting factor expression might also account for the differences in corepressor alternative splicing within different tissues and at different developmental stages in the same species.
Alternative splicing of these corepressors at other mRNA sites and in non-vertebrates. Our above studies were focused on the alternative splicing events previously reported to occur in Xenopus and/or mice (the splicing events that altered the function or presence of the RID domains were of particular interest). To explore if there might be alternative splicing at yet additional locations in certain species but not previously observed in the Xenopus and mammalian studies, we next employed additional PCR primers that surveyed the entire SMRT and NCoR open reading frames, and thus would detect any previously undescribed alternative splicing. We initially studied Danio given that this telostat is the most taxonomically distant from the species employed in our prior published studies.
Notably in Danio there was evidence of additional alternative splicing events located between NCoR bases 2589 and 3594 and SMRT bases 3134 and 3978 (Additional file 1: Figure S3, NCoR primer pair 3 and SMRT primer pair 4). The ratios of the alternatively spliced variants at these locations differed between different Danio organs (e.g., Additional file 1: Figure S3A and B). These Danio alternative splice sites were not reported in prior studies of mammalian or Xenopus alternative splicing. They map to an NCoR region that that bears a domain implicated in the Siah2-mediated degradation of NCoR, the binding of unacetylated histone H4, and to a SMRT region previously shown to contribute in an unresolved manner to the overall repression phenotype [21, 40]. In addition to these novel SMRT and NCoR variants, the alternative splicing of SMRT exon 40b+/b- already described above for Danio brain was again evident when using the extended SMRT survey primer pairs 7 or 8 (which overlap the relevant region; Additional file 1: Figure S3A). The biological consequences of these newly recognized alternative splicing events will be examined in future experiments.
To further extend this type of analysis beyond vertebrates, we next examined the alternative splicing of SMRTER in the arthropod Drosophila melanogaster. SMRTER, and its nematode ortholog GEI-8, bear notable, although limited, sequence relatedness to the deuterostoma SMRT and NCoR corepressors (e.g., ). By surveying the Drosophila SMRTER open reading frame as above, we identified two regions, between nucleotide 6275–6779 and between 6760 and 7302, that were alternatively spliced (Additional file 1: Figure S4). The former removed approximately 500 bps and the second added approximately 150 bps. There is no observable sequence relatedness between these alternatively spliced regions in SMRTER and the vertebrate SMRT or NCoR, leaving the functional impact of these alternative splices difficult to predict. Nonetheless we conclude that alternative splicing of the SMRT/NCoR/SMRTER family exists widely throughout phylogeny, but differs in its details across different taxa.
NCoR and SMRT are thought to have arisen from an ancestral duplication and divergence of a common precursor locus, with this divergence occurring just prior to the evolutionary appearance of vertebrates. Distinct loci for NCoR and for SMRT (also known as NCoR1 and NCoR2) can be identified in virtually every vertebrate genome sequenced to date, spanning phylogeny from the cartilaginous and bony fish to the primates (e.g., [17, 39]). There is an approximate 36 % overall amino acid identity between NCoR and SMRT, and NCoR and SMRT play both unique and shared roles in biology. Loci with extended sequence relatedness to both NCoR and SMRT also exist in the genomes of the non-vertebrate Chordata and Echinodermata, but apparently as a single “combined” locus in each genome . Therefore the initial duplication and divergence of distinct SMRT and NCoR corepressor loci likely occurred early during the origins of the vertebrates [17, 21]. Whether this was a localized gene duplication or reflected instead the more extensive whole genomic duplications proposed for early vertebrate evolution in the “2R” hypothesis  is unclear, although a preliminary chromosomal synteny search appears to favor the former hypothesis (data not shown).
Even more distant phyla, such as the arthopoda and nematoda, also contain single loci (e.g., SMRTER and GEI-8) related to both vertebrate SMRT and NCoR. However the sequence relatedness of these more distant loci is limited to N-terminal regions, and lacks the C-terminal architecture and CoRNR box sequences characteristic of deuterosomatia NCoR and SMRT ; thus the precise evolutionary or functional relationship of the former to the latter is not clear.
Alternative splicing of SMRT occurs in the teleost Danio, in the bird Gallus, and in the reptile Trachemys, but this is relatively minimal at the RNA sites previously reported in mammals or amphibians. A very modest amount of production of the SMRT exon 40b + variant was detected in Danio at the 84 h stage (i.e., “hatchlings” that display an overall adult-like morphology). Dissection of Danio adults indicates that this 40b + SMRT variant is found primarily in the brain, in parallel to the enhanced abundance of this variant in the murine brain. The Danio SMRT 40b + variant however represents only a small fraction of the total SMRT transcripts in brain and was not detected in any other organ examined. The 40b + SMRT exon contains an S3-CoRNR box whereas the 40b- exon does not. Thus the 40b + exon SMRT variants can encode up to three RIDs, whereas the 40b- exon SMRT variants contains no more than two RIDS. The murine SMRT40b+/b- variants are known to display greatly different preferences for different nuclear receptors and to play distinct biological roles [20, 22, 23, 26, 27]. In contrast, no alternative spliced variants at SMRT at exons 28b + or 47b-, or at NCoR exons 28+, 37b, or 45a, are detected in Danio.
Corepressors in the bird Gallus also display limited alternative slicing, but at SMRT exon 47 (producing 47b+, b-, and – variants) and not at SMRT exon 40b. We have shown that proteins derived from the three different SMRT exon 47 variants also possess distinct affinities for different nuclear receptors . Alternative splicing in the reptile, Trachemys, exhibits both the SMRT exon 40b+/b- and SMRT exon 47b+/b−/− splice variants, which further diversify the potential functions of SMRT in these taxa compared to Danio or Gallus.
Both shared and unique patterns of alternative splicing of SMRT are observed in the amphibian, Xenopus. A high abundance of the 40b + SMRT variant is observed in Xenopus, as well as the additional alternative splicing event at SMRT exon 47. Interestingly, although all three exon 47 variants are expressed in Gallus and Trachemys the exon 47- variant is lacking in Xenopus. Similarly, alternatively spliced SMRT variants which alter the distance between RID1 and 2 (exon 44+/− and 45+/−) are observed in Xenopus but are not seen in any other lineage we examined (these too are anticipated to impact on SMRT affinity for its different transcription factor partners). Analogous patterns of alternative splicing sites is also observed at earlier stages of Xenopus development.
We suggest that the divergence of amphibia, reptilia, and ava from the teleost lineage was coincident with a more extensive use of the SMRT 40b + exon and/or additional alternative splicing at SMRT exon 47, both of which significantly diversify the biochemistry and transcription partner preferences displayed by this corepressor. Notably these three lineages express nuclear receptors, e.g., TRs, PPARs, and LXRs, that have been shown to exhibit different interactions with the corresponding corepressor splice variants (as tested in vitro using the mammalian versions) [22, 23].
The alternative splicing of NCoR is restricted to placental mammals and appears to have arisen later in evolution than that of SMRT. Alternative splicing of SMRT was observed in both the placental and marsupial lineages examined, although to different extents among the different species. However unlike SMRT, alternative splicing of NCoR was not observed in non-placental mammals; of the 3 different NCoR alternative splice sites that are utilized in the placental mammals, the alternative splicing of NCoR at exon 37b-/b + is of particular interest. Although restricted to the placental mammals, this alternative splicing of NCoR alters the structure of the encoded corepressor in a manner remarkably similar to the alternative splicing that occurs in virtually all vertebrates at SMRT exon 40b+/b-. Both the NCoR and SMRT splicing events insert or remove a third RID region from the encoded protein, both display closely related tissue distributions (including particularly high abundances of the RID3-containing variants in brain and testis), and both produce proteins with overlapping preferences for certain transcription factor partners [23, 26, 27].
Despite these commonalities, however, the precise sites of the splices, the length of the alternative spliced exons, and the precise amino acid compositions of these exons differ substantially between NCoR and SMRT . Although we do not rule out the possibility that this alternative splicing occurred prior to the SMRT/NCoR divergence and that NCoR subsequently lost this ability in several lineages , we view the data overall as favoring instead the proposal that the NCoR N37b+/b- and SMRT S40b+/b- alternative splice sites arose separately at different times in evolution and are the outcome of a convergent evolutionary process. We speculate that this convergent evolution may have been driven by functional requirements unique to the placental mammals that favored diversification of NCoR into RID3 −/+ variants in a manner similar, but operating in addition, to that which favored diversification of SMRT into its RID3−/+ variants during earlier vertebrate evolution . Consistent with this hypothesis, NCoR 37b+/b- splicing regulates adipose tissue function, lipid accumulation, and glucose utilization in ways that SMRT 40b+/b- splicing does not [24, 29]. The effects of diet on NCoR 37b+/b- and SMRT 40b+/b- splicing also differ [24, 29]. Given known differences in these energy storage and utilization processes in placental mammals versus other vertebrates, it is tempting to speculate that the acquisition of alternative splicing at the NCoR 37b+/b- site in placental mammals may in part reflect a requirement for more sophisticated regulation of these metabolic pathways in the former.
Also of relevance: SMRT is alternatively spliced at exons 47b+/47b-/47- so as to alter the presence and function of its RID 1 domain , yet no comparable alternative splicing is detected in NCoR in any lineage examined. The acquisition of this alternative splicing event by SMRT but not by NCoR is therefore likely an example of a divergent, rather than convergent, evolutionary event that occurred after duplication of the common ancestral corepressor gene.
Novel sites of alternative splicing of SMRT and NCoR in different species and in Drosophila SMRTER. We also detected previous undescribed alternative splice sites within the coding regions of both Danio SMRT and NCoR, as well as of Drosophila SMRTER. These additional alternative splicing sites map outside of the RIDs themselves and affect regions that may influence the assembly of the corepressor holocomplex, histone binding, or functions yet to be established. Our results therefore indicate that alternative corepressor splicing is a common theme among a wide variety of metazoans with utilization of these splice sites differing from species to species.
Limitations of our methodology. As detailed in the Materials and Methods, our approach was highly reproducible, with little or no variation detected among different individuals within a species. Our methodology did require sufficiently annotated genomic sequences to allow design of RT-PCR primers, as well as a source of adequately intact RNA. As a result, several taxa that might have extended our analysis, such as monotremes, were unavailable to us, and we were restricted to examining only a limited number of species from a given taxonomic order. Certain corepressor splice variants may only exist within specific tissues or organs that were not tested in this study (although we typically see greater differences in the sites of alternative splicing between species than between the different tissues/organs within a single species; this manuscript and data not shown). Finally we can not fully rule out the presence of splice variants that might be produced below detectable levels (~2 % or less) or that might lie outside the specific regions analyzed here.
Despite these limitations, our experiments nonetheless elucidated key aspects of the evolution of the previously-reported alternative splice sites in these corepressors, particularly the alternate splicing events within the RIDs that define the interactions of these corepressors with many of their transcription factor partners. Full understanding of the biological implications of the novel corepressor splice sites newly detected by our experiments in Danio and in Drosophila will require extensive future biochemical and taxonomic analysis.
It is notable that alternative splicing, which is frequently viewed as a “polishing” step in gene expression that only modestly alters function, has in fact dramatic, even opposing effects on corepressor function. Splicing therefore appears to be a fundamental process by which a limited number of genes are diversified to yield distinct protein products that exert distinct functions in different tissues and at different times in development and differentiation. This manuscript reveals that different species employ different strategies in their alternative splicing of SMRT and of NCoR. Rather than viewing this as a neutral variation we favor the interpretation that this species-specific utilization of alternative corepressor splicing reflects natural selection and serves to customize alternative corepressor splicing to the biological requirements of each species. We base this view on several observations. (a) The alternative splice sites are located non-randomly such that they incorporate or exclude many of the important functional domains previously identified within the corepressor open reading frame. As a consequence the individual corepressor splice variants exhibit different molecular properties, favor different transcription factor partners, and play different roles in essential biological processes. The ability of a species to express a given alternative corepressor splice variant is therefore expected to represent an important selectable trait. (b) Consistent with this concept, the relative abundances of the different splice variants within a given species are actively controlled in response to tissue type, differentiation signals, and extracellular environment, further suggesting that the different variants exert biologically significant, yet distinct, functions that are coordinated so as to maintain the overall health and fitness of the organism. (c) Alternative splicing that adds or removes the S3/N3 RIDs appears to have arisen separately in SMRT and NCoR by convergent evolution, suggesting that these alternative splicing events were under selective pressure so as to generate similar (but not identical) variants of each corepressor paralog.
We are deeply indebted to Liming Liu and Dr. Betty Ma for truly superb technical assistance and to Andres Baumier, Olga Barmina, Hannah Bishop, Bruce Draper, David Furlow, Michael Kirmiz, Artyom Kopp, James Trimmer, and Eric Velazquez for generously providing biological samples from various species and expert advice. All animals were maintained and samples obtained using approved U.C. Davis protocol. The work was supported by grant #7-12-BS-151 from the American Diabetes Association and from internal funding providing by the Department of Microbiology and Molecular Biology and from the College of Biological Sciences. Neither funding source was involved in he design, collection, analysis, or interpretation of data, or in the writing of or the decision to submit this manuscript for publication.
Availability of data and materials
The data supporting the results of this article are included within the article (and its additional supplemental files).
MLP carried out the experimental studies and drafted the manuscript. CAS and MLG provided essential clones, helped devise and optimize the alternative splicing assays, and assisted in editing the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
All animals were maintained and euthanized using University of California at Davis authorized protocols and with the written approval of the University of California at Davis Animal Use committee; samples of human materials were previously collected and encoded to ensure anonymity, allowing them to be defined as exempt from regulation by the U. C. Davis Internal Review Board.
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- Edwards DP. The role of coactivators and corepressors in the biology and mechanism of action of steroid hormone receptors. J Mammary Gland Biol Neoplasia. 2000;5(3):307–24.View ArticlePubMedGoogle Scholar
- Feige JN, Auwerx J. Transcriptional coregulators in the control of energy homeostasis. Trends Cell Biol. 2007;17(6):292–301.View ArticlePubMedGoogle Scholar
- Hu X, Lazar MA. Transcriptional repression by nuclear hormone receptors. Trends Endocrinol Metab. 2000;11(1):6–10.View ArticlePubMedGoogle Scholar
- Jepsen K, Rosenfeld MG. Biological roles and mechanistic actions of co-repressor complexes. J Cell Sci. 2002;115(Pt 4):689–98.PubMedGoogle Scholar
- Lonard DM, O’Malley BW. Nuclear receptor coregulators: judges, juries, and executioners of cellular regulation. Mol Cell. 2007;27(5):691–700.View ArticlePubMedGoogle Scholar
- Ordentlich P, Downes M, Evans RM. Corepressors and nuclear hormone receptor function. Curr Top Microbiol Immunol. 2001;254:101–16.PubMedGoogle Scholar
- Rosenfeld MG, Lunyak VV, Glass CK. Sensors and signals: a coactivator/corepressor/epigenetic code for integrating signal-dependent programs of transcriptional response. Genes Dev. 2006;20(11):1405–28.View ArticlePubMedGoogle Scholar
- Savkur RS, Bramlett KS, Clawson D, Burris TP. Pharmacology of nuclear receptor-coregulator recognition. Vitam Horm. 2004;68:145–83.View ArticlePubMedGoogle Scholar
- Astapova I, Hollenberg AN. The in vivo role of nuclear receptor corepressors in thyroid hormone action. Biochim Biophys Acta. 2013;1830(7):3876–81.View ArticlePubMedGoogle Scholar
- Lazar MA. Nuclear receptor corepressors. Nucl Recept Signal. 2003;1, e001.View ArticlePubMedPubMed CentralGoogle Scholar
- Lee JW, Lee YC, Na SY, Jung DJ, Lee SK. Transcriptional coregulators of the nuclear receptor superfamily: coactivators and corepressors. Cell Mol Life Sci. 2001;58(2):289–97.View ArticlePubMedGoogle Scholar
- Mottis A, Mouchiroud L, Auwerx J. Emerging roles of the corepressors NCoR1 and SMRT in homeostasis. Genes Dev. 2013;27(8):819–35.View ArticlePubMedPubMed CentralGoogle Scholar
- Perissi V, Jepsen K, Glass CK, Rosenfeld MG. Deconstructing repression: evolving models of co-repressor action. Nat Rev Genet. 2010;11(2):109–23.View ArticlePubMedGoogle Scholar
- Stanya KJ, Kao HY. New insights into the functions and regulation of the transcriptional corepressors SMRT and N-CoR. Cell Div. 2009;4:7.View ArticlePubMedPubMed CentralGoogle Scholar
- Watson PJ, Fairall L, Schwabe JW. Nuclear hormone receptor co-repressors: structure and function. Mol Cell Endocrinol. 2012;348(2):440–9.View ArticlePubMedGoogle Scholar
- Privalsky ML. The role of corepressors in transcriptional regulation by nuclear hormone receptors. Annu Rev Physiol. 2004;66:315–60.View ArticlePubMedGoogle Scholar
- Short S, Peterkin T, Guille M, Patient R, Sharpe C. Short linear motif acquisition, exon formation and alternative splicing determine a pathway to diversity for NCoR-family co-repressors. Open Biol 2015;5(8). doi:10.1098/rsob.150063.
- Mikolas P, Kollarova J, Sebkova K, Saudek V, Yilma P, Kostrouchova M, Krause MW, Kostrouch Z, Kostrouchova M. GEI-8, a homologue of vertebrate nuclear receptor corepressor NCoR/SMRT, regulates gonad development and neuronal functions in Caenorhabditis elegans. PLoS One. 2013;8(3), e58462.View ArticlePubMedPubMed CentralGoogle Scholar
- Tsai CC, Kao HY, Yao TP, McKeown M, Evans RM. SMRTER, a Drosophila nuclear receptor coregulator, reveals that EcR-mediated repression is critical for development. Mol Cell. 1999;4(2):175–86.View ArticlePubMedGoogle Scholar
- Faist F, Short S, Kneale GG, Sharpe CR. Alternative splicing determines the interaction of SMRT isoforms with nuclear receptor-DNA complexes. Biosci Rep. 2009;29(3):143–9.View ArticlePubMedGoogle Scholar
- Goodson M, Jonas BA, Privalsky MA. Corepressors: custom tailoring and alterations while you wait. Nucl Recept Signal. 2005;3, e003.View ArticlePubMedPubMed CentralGoogle Scholar
- Goodson ML, Jonas BA, Privalsky ML. Alternative mRNA splicing of SMRT creates functional diversity by generating corepressor isoforms with different affinities for different nuclear receptors. J Biol Chem. 2005;280(9):7493–503.View ArticlePubMedPubMed CentralGoogle Scholar
- Goodson ML, Mengeling BJ, Jonas BA, Privalsky ML. Alternative mRNA splicing of corepressors generates variants that play opposing roles in adipocyte differentiation. J Biol Chem. 2011;286:44988–99.View ArticlePubMedPubMed CentralGoogle Scholar
- Goodson ML, Young B, Synder CA, Shroeder AC, Privalsky ML. Alteration of NCoR corepressor splicing in mice causes increased body weight and hepatosteatosis without glucose intolerance. Mol Cell Biol. 2014;34(22):4104–14.View ArticlePubMedPubMed CentralGoogle Scholar
- Malartre M, Short S, Sharpe C. Alternative splicing generates multiple SMRT transcripts encoding conserved repressor domains linked to variable transcription factor interaction domains. Nucleic Acids Res. 2004;32(15):4676–86.View ArticlePubMedPubMed CentralGoogle Scholar
- Mengeling BJ, Goodson ML, Bourguet W, Privalsky ML. SMRTepsilon, a corepressor variant that interacts with a unique subset of nuclear receptors and displays a high specificity for retinoic acid receptors. Mol Cell Endocrinol. 2012;351:306–16.View ArticlePubMedPubMed CentralGoogle Scholar
- Mengeling BJ, Phan TQ, Goodson ML, Privalsky ML. PML-RARalpha and PLZF-RARalpha oncoproteins display an altered recruitment and release of specific corepressor variants. J Biol Chem. 2011;286(6):4236–47.View ArticlePubMedGoogle Scholar
- Short S, Malartre M, Sharpe C. SMRT has tissue-specific isoform profiles that include a form containing one CoRNR box. Biochem Biophys Res Commun. 2005;334(3):845–52.View ArticlePubMedGoogle Scholar
- Snyder CA, Goodson ML, Schroeder AC, Privalsky ML. Regulation of corepressor alternative mRNA splicing by hormonal and metabolic signaling. Mol Cell Endocrinol. 2015;413:228–35.View ArticlePubMedPubMed CentralGoogle Scholar
- Malartre M, Short S, Sharpe C. Xenopus embryos lacking specific isoforms of the corepressor SMRT develop abnormal heads. Dev Biol. 2006;292(2):333–43.View ArticlePubMedGoogle Scholar
- Chen JD, Evans RM. A transcriptional co-repressor that interacts with nuclear hormone receptors. Nature. 1995;377(6548):454–7.View ArticlePubMedGoogle Scholar
- Downes M, Burke LJ, Bailey PJ, Muscat GE. Two receptor interaction domains in the corepressor, N-CoR/RIP13, are required for an efficient interaction with Rev-erbA alpha and RVR: physical association is dependent on the E region of the orphan receptors. Nucleic Acids Res. 1996;24(22):4379–86.View ArticlePubMedPubMed CentralGoogle Scholar
- Heinzel T, Lavinsky RM, Mullen TM, Söderstrom M, Laherty CD, Torchia J, Yang WM, Brard G, Ngo SD, Davie JR, et al. A complex containing N-CoR, mSin3 and histone deacetylase mediates transcriptional repression [see comments]. Nature. 1997;387(6628):43–8.View ArticlePubMedGoogle Scholar
- Ordentlich P, Downes M, Xie W, Genin A, Spinner NB, Evans RM. Unique forms of human and mouse nuclear receptor corepressor SMRT. Proc Natl Acad Sci U S A. 1999;96(6):2639–44.View ArticlePubMedPubMed CentralGoogle Scholar
- Park EJ, Schroen DJ, Yang M, Li H, Li L, Chen JD. SMRTe, a silencing mediator for retinoid and thyroid hormone receptors-extended isoform that is more related to the nuclear receptor corepressor. Proc Natl Acad Sci U S A. 1999;96(7):3519–24.View ArticlePubMedPubMed CentralGoogle Scholar
- Sande S, Privalsky ML. Identification of TRACs (T3 receptor-associating cofactors), a family of cofactors that associate with, and modulate the activity of, nuclear hormone receptors. Mol Endocrinol. 1996;10(7):813–25.PubMedGoogle Scholar
- Seol W, Mahon MJ, Lee YK, Moore DD. Two receptor interacting domains in the nuclear hormone receptor corepressor RIP13/N-CoR. Mol Endocrinol. 1996;10(12):1646–55.PubMedGoogle Scholar
- Zamir I, Harding HP, Atkins GB, Hörlein A, Glass CK, Rosenfeld MG, Lazar MA. A nuclear hormone receptor corepressor mediates transcriptional silencing by receptors with distinct repression domains. Mol Cell Biol. 1996;16(10):5458–65.View ArticlePubMedPubMed CentralGoogle Scholar
- Cunningham F, Amode MR, Barrell D, Beal K, Billis K, Brent S, Carvalho-Silva D, Clapham P, Coates G, Fitzgerald S, et al. Ensembl 2015. Nucleic Acids Res. 2015;43(Database issue):D662–9.View ArticlePubMedGoogle Scholar
- Zhang J, Guenther MG, Carthew RW, Lazar MA. Proteasomal regulation of nuclear receptor corepressor-mediated repression. Genes Dev. 1998;12(12):1775–80.View ArticlePubMedPubMed CentralGoogle Scholar
- Canestro C, Albalat R, Irimia M, Garcia-Fernandez J. Impact of gene gains, losses and duplication modes on the origin and diversification of vertebrates. Semin Cell Dev Biol. 2013;24(2):83–94.View ArticlePubMedGoogle Scholar