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Fig. 4 | BMC Evolutionary Biology

Fig. 4

From: Recent horizontal transfer, functional adaptation and dissemination of a bacterial group II intron

Fig. 4

Splicing efficiency of Ef.PcfG and Ll.LtrB in L. lactis. a Group II intron splicing efficiency assessed by poisoned primer extension assay. This assay monitors splicing efficiency by comparing the relative abundance of precursor and ligated exons from total RNA extracts. A P32-labeled primer (Additional file 1: Table S2) complementary to exon 2 was extended from both the precusor and the ligated exons in the presence of a high concentration of ddCTP. Since the sequence of the two RNAs are different after the exon 2 junction the first G residue encountered is at a different distance from the primer generating differently sized bands for the precursor (53 nts) and the ligated exons (51 nts). b The splicing efficiency (%) of Ef.PcfG and Ll.LtrB was assessed from their wild-type and exchanged flanking exons and calculated as the relative intensity of the ligated exons and precursor bands (ligated exons/precursor + ligated exons)

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