Alternative evolution of a spheroidal colony in volvocine algae: developmental analysis of embryogenesis in Astrephomene (Volvocales, Chlorophyta)
© The Author(s). 2016
Received: 12 August 2016
Accepted: 7 October 2016
Published: 9 November 2016
Volvocine algae, which range from the unicellular Chlamydomonas to the multicellular Volvox with a germ–soma division of labor, are a model for the evolution of multicellularity. Within this group, the spheroidal colony might have evolved in two independent lineages: Volvocaceae and the goniacean Astrephomene. Astrephomene produces spheroidal colonies with posterior somatic cells. The feature that distinguishes Astrephomene from the volvocacean algae is lack of inversion during embryogenesis; the volvocacean embryo undergoes inversion after successive divisions to orient flagella toward the outside. The mechanisms of inversion at the molecular and cellular levels in volvocacean algae have been assessed in detail, particularly in Volvox carteri. However, embryogenesis in Astrephomene has not been subjected to such investigations.
This study relied on light microscopy time-lapse imaging using an actively growing culture of a newly established strain to conduct a developmental analysis of Astrephomene as well as to perform a comparison with the similar spheroidal volvocacean Eudorina. During the successive divisions involved in Astrephomene embryogenesis, gradual rotation of daughter protoplasts resulted in movement of their apical portions toward the embryonic posterior, forming a convex-to-spheroidal cell sheet with the apical ends of protoplasts on the outside. Differentiation of the posterior somatic cells from the embryo periphery was traced based on cell lineages during embryogenesis. In contrast, in Eudorina, the rotation of daughter protoplasts did not occur during successive cell divisions; however, inversion occurred after such divisions, and a spheroidal embryo was formed. Indirect immunofluorescence microscopy of basal bodies and nuclei verified this difference between Astrephomene and Eudorina in the movement of embryonic protoplasts.
These results suggest different tactics for spheroidal colony formation between the two lineages: rotation of daughter protoplasts during successive cell divisions in Astrephomene, and inversion after cell divisions in Eudorina. This study will facilitate further research into the molecular and genetic mechanisms of the parallel evolution of the spheroidal colony in volvocine algae.
The evolutionary origin of multicellularity is a topic of increasing interest. The evolution from unicellular to multicellular organisms is one of the evolutionary transitions in individuality (i.e., the integration of individuals into a new higher-level individual), which bring about diversification and the hierarchical organization of the biosphere . The emergence of multicellularity occurred in at least 25 eukaryotic lineages independently . However, almost all the multicellular lineages lack extant transitional states from unicellular ancestors, which hampers determination of the initial stages of multicellularity.
Recent phylogenetic studies of the volvocine lineage have suggested that the spheroidal colony might have evolved from a flattened ancestor in two independent lineages: Volvocaceae and the goniacean Astrephomene [4, 5, 11]. Although the spheroidal colonies of these two lineages resemble each other in terms of their external morphology, there is a crucial difference between them in modes of colonial development or embryogenesis. Volvocaceae species undergo the drastic morphogenetic process known as inversion, whereas Astrephomene species do not [4, 5, 11].
Inversion is common among volvocacean species [4, 5, 11]. Each reproductive cell or gonidium undergoes successive cell divisions to form a concave-to-cup-shaped embryo or plakea composed of a single cell layer. Immediately after the successive divisions (“palintomy” or rapid multiple fissions without cell growth ), the cell layer is inside out compared with the adult configuration—the apical ends of the embryo protoplasts, in which basal bodies are localized and flagella are formed, are oriented toward the interior of the plakea. Therefore, the embryo undergoes inversion, during which the cell layer is inverted to form a spheroidal daughter colony with the apical ends and flagella of daughter protoplasts positioned outside. This process enables appropriate locomotion of spheroidal colonies of the Volvocaceae. The mechanism of inversion has been investigated extensively at the cellular and molecular levels using a model species, Volvox carteri [13–17]. Compared with that of Volvox, inversion in other volvocacean genera—such as Pandorina, Eudorina, and Pleodorina—is relatively simple [18–23]. Inversion in these volvocacean species has been examined by light microscopy time-lapse imaging  as well as by electron microscopy [18–20].
However, the developmental tactic of the volvocacean species is not applicable to other lineages of volvocine algae that form spheroidal colonies (Fig. 1). Astrephomene has 32- or 64-celled spheroidal colonies resembling those of some volvocacean species, but there is a difference in the distribution of somatic cells; Astrephomene has two or four somatic cells distributed only in the posterior pole of the colony [24, 25] whereas volvocacean species lack somatic cells or have somatic cells distributed in the anterior pole of the colony. The feature of Astrephomene that distinguishes them from the Volvocaceae is the lack of inversion during embryogenesis; each reproductive cell in a colony divides successively to form a spheroidal daughter colony [24, 26]. The embryogenesis of Astrephomene has been visualized by light microscopy in previous studies [24–26]. However, successive observations by light microscopy time-lapse imaging and cell-based studies of spheroidal colony formation in Astrephomene have not been performed; thus, the mechanism underlying the formation of a spheroidal colony without inversion is unclear.
The present study was undertaken to evaluate the cellular or subcellular mechanisms underlying the formation of spheroidal colonies of Astrephomene; to this end, we used light microscopy time-lapse imaging of an actively growing culture of a newly established strain and compared it with that of a volvocacean Eudorina, which has a similar cell number and colony size. The developmental events observed by time-lapse imaging were verified at the subcellular level by tracing the behavior of the basal bodies and nuclei of daughter protoplasts by indirect immunofluorescence microscopy. These results will facilitate further research into the cellular and molecular bases of the parallel evolution of spheroidal colony formation within the volvocine algae.
Time-lapse imaging of embryogenesis in Astrephomene
Additional file 1: Movie S1. Time-lapse analysis of anterior-lateral view of embryogenesis in Astrephomene. Note the rotation of daughter protoplasts during successive cell divisions. Scale bar: 5 μm, 900x speed. (AVI 2600 kb)
Additional file 2: Movie S2. Time-lapse analysis of lateral view of embryogenesis in Astrephomene. Scale bar: 5 μm, 900x speed. (AVI 2160 kb)
Additional file 3: Movie S3. Time-lapse analysis of posterior-lateral view of embryogenesis in Astrephomene. Note the change of direction in four posterior somatic cells after successive cell divisions. Scale bar: 5 μm, 900x speed. (AVI 2420 kb)
Additional file 4: Movie S4. Time-lapse analysis of posterior view of embryogenesis in Astrephomene. Note the posterior gap surrounding by 4 cells (Fig. 2j; 1 pp, 2 pp, 1 pp’ and 2 pp’ cells) closed just after the sixth cell divisions. Scale bar: 5 μm, 900x speed. (AVI 2510 kb)
Based on the present time-lapse imaging, gradual rotation of daughter protoplasts was observed during successive divisions. The first two cleavages did not involve rotation of protoplasts and were similar to those in other volvocine algae ; this resulted in the generation of a four-celled embryo (Fig. 2a, f), which is typical of the colonies of volvocine algae , with two diagonal protoplasts attached to each other in the center (Fig. 2f). The rotation of daughter protoplasts was observed after the second division and occurred in conjunction with movement of the outer surface of the cell layer or the apical ends of daughter protoplasts toward the posterior of the embryo. In an optical section from the lateral side of the embryo, daughter protoplasts were positioned at the right side of the embryo rotated clockwise, whereas protoplasts at the left side were rotated counterclockwise (Fig. 3a, b). Immediately prior to the third cleavage, each daughter protoplast underwent slight rotation, which resulted in the formation of a concave eight-celled embryo with outside apical ends after the third cleavage (Fig. 2b, g). The rotation of daughter protoplasts was most marked immediately before the fourth cleavage (Fig. 3a–c); the apical ends of protoplasts, which frequently exhibited acute apices opposite pale-green chloroplasts, moved from the anterior to the posterior direction of the embryo (Fig. 3a, b). Moreover, the angles between the fourth cleavage planes and the longitudinal axis of the embryo were larger than those for the planes of the third cleavage. A hemispherical 16-celled embryo was formed after the fourth cleavage (Figs. 2c, h and 3c). Protoplasts underwent lesser rotations prior to the fifth cleavage than before the fourth cleavage, which resulted in an almost spheroidal 32-celled embryo with a small gap at the posterior pole (Fig. 2d, i). A very slight rotation of the protoplasts occurred before the sixth cleavage, resulting in the formation of a 64-celled embryo with a small gap at the posterior pole (Fig. 2e, j).
Immediately after the sixth cleavage, daughter protoplasts narrowed and commenced to produce flagella, and the posterior gap closed (Figs. 2j and 3d, e). Daughter protoplasts subsequently became flattened, and the hollow spheroidal daughter colony expanded (Fig. 3d, e). Simultaneously, the orientation of the longitudinal axes of the four cells at the posterior pole of the embryo changed from lateral to posterior, and their apical ends became protruded (Fig. 3f). These four cells were elongate-ovoid or ellipsoidal in shape and slightly larger than the other daughter cells. The most posterior position and orientation of the longitudinal axes of the four cells corresponded to those in posterior somatic cells of mature vegetative colonies [24, 25] (see also Additional file 5: Figure S1); therefore, these four cells likely become somatic cells at maturity.
Based on images of the developing embryos obtained using different optical sections and angles, the cell lineage of somatic cells was traced. The pattern of cell divisions was 180° rotationally symmetrical about the longitudinal axis of the embryo, and the origins of four posterior somatic cells (Fig. 2e, j; 1 pp, 2 pp, 1 pp’, and 2 pp’ cells) were determined; the somatic cells were derived from two diagonally opposite daughter protoplasts in four-celled embryos (Fig. 2a, f; A and A’ cells) and through two opposite pairs of adjoining protoplasts at the periphery of 16-celled embryos (Fig. 2c, h; A1, A2, A1’ and A2’ cells).
Additional file 6: Movie S5. Time-lapse analysis of anterior-lateral view of embryogenesis in Eudorina. Note absence of rotation of daughter protoplasts during successive cell divisions and inversion with stalk formation after cell divisions. Scale bar: 5 μm, 900x speed. (AVI 2440 kb)
Indirect immunofluorescence microscopy of basal bodies and nuclei
The cellular behavior observed by light microscopy time-lapse imaging was verified at the subcellular level by indirect immunofluorescence microscopy of basal bodies and nuclei. For immunostaining of basal bodies, an antibody against Chlamydomonas SAS-6, which is localized in mature and immature basal bodies , was reacted with fixed embryos; thus, two basal bodies and two pro-basal bodies in each cell were observed as four dots. An anti-histone antibody was reacted with the samples at the same time, allowing simultaneous visualization of basal bodies and nuclei. The localization of basal bodies and nuclei was used to identify the longitudinal axis of daughter protoplasts during embryogenesis.
Using light microscopy time-lapse imaging of the embryogenesis of Astrephomene with a high temporal resolution (1 min) and several views, we demonstrated the dynamic cellular events, rotation of daughter protoplasts between cell divisions, and drastic change in the orientation of the posterior somatic cells after successive cell divisions. Time-lapse observation was required as the intervals between cell divisions were > 20 min, and rotational movement was slow. These results were verified by the indirect immunofluorescence microscopy of basal bodies and nuclei.
Comparison of the cellular events during the embryogenesis of Astrephomene with those in that of volvocacean Eudorina revealed two distinct mechanisms of spheroidal colony formation (Fig. 7). For the appropriate locomotion of the spheroidal colony, the basal bodies of daughter protoplasts in the embryos of both lineages must be oriented toward the outside. In Astrephomene, the rotation of daughter protoplasts with the movement of basal bodies occurred between cell divisions, resulting in the formation of a convex-to-spheroidal cell layer with basal bodies outside (Figs. 3a–c and 5a–c). In contrast, we did not observe rotation of daughter protoplasts or movement of basal bodies during successive cell divisions in Eudorina (Figs. 4a–c and 6a–c). Inversion then occurred to form a spheroidal daughter colony with basal bodies outside (Figs. 4d–f and 6d–f), as in other members of the Volvocaceae . Thus, the following mechanisms of spheroidal colony formation seem to have evolved in the two lineages: gradual rotation of protoplasts during successive divisions in Astrephomene, and inversion after successive divisions in the Volvocaceae.
This study also demonstrated the cell lineages of posterior somatic cells (Fig. 2) and their change of direction toward the posterior pole after successive cell divisions (Fig. 3d–f). The volvocacean species Pleodorina californica has somatic cells at the anterior region in a spheroidal colony. These anterior somatic cells are derived from the daughter protoplasts in the center of a plakea during embryogenesis . In Astrephomene, the posterior somatic cells were derived from the periphery of the embryo. These phenomena show that the correspondence of the central–peripheral polarity in embryos to the anterior–posterior axis in mature spheroidal colonies is conserved between the two lineages.
Using light microscopy time-lapse imaging and indirect immunofluorescence microscopy of basal bodies and nuclei, we identified a difference in the cellular mechanism of spheroidal colony formation between Astrephomene and the Volvocaceae in the present study. The major cellular mechanisms of spheroid colony formation in Astrephomene and the Volvocaceae are rotation of the protoplast during successive cell divisions and inversion, respectively. However, these two mechanisms might be related to the homologous molecular mechanisms discussed above. Further research will reveal the molecular and genetic bases of the parallel evolution of the spheroidal colony in volvocine algae.
Strains and culture conditions
The Astrephomene strains established in previous research  showed abnormal morphology (not shown), possibly due to their long-term maintenance. Thus, a new strain of Astrephomene suitable for developmental observation was established in the present study. Astrephomene gubernaculifera strain 2014-1002-YkAs8 was isolated from a soil sample collected from a rice paddy field (35° 23’23.1” N, 140°01’36.2” E) in Yokota, Sodegaura, Chiba Prefecture, Japan on July 21, 2014. The dried soil was rewetted with boiled pea (Pisum sativum) and its extract in Petri dishes (90 × 20 mm) and incubated at 25 °C on a 12-h light/12-h dark schedule under cool-white fluorescent lamps at an intensity of 50–90 μmol · m−2 · s−1. After 3–4 days, vegetative colonies appeared in the Petri dishes. Clones were established using the pipette-washing method  and grown in 10 mL of VTAC medium  in screw-capped tubes (18 × 150 mm). The culture was maintained at 25 °C on a 12-h light/12-h dark schedule as described above. Species identification was carried out based on morphological and molecular data (Additional file 5: Figures S1 and S2).
For comparison with volvocacean species with similar colonial organization, Eudorina sp. strain 2010-623-F1-E8 was used. This is the F1 strain of Eudorina sp. NIES-2734 and NIES-2735  and was used in a previous study .
For light microscopy time-lapse imaging, both species were grown in screw-capped tubes (18 × 150 mm) containing 10 mL VTAC medium under the culture conditions described above. For indirect immunofluorescent microscopy, to improve the synchrony, cultures were grown in silicon-capped 500 mL Erlenmeyer flasks containing 250 mL VTAC medium with aeration at 32 °C on a 16-h light/8-h dark schedule under cool-white fluorescent lamps at an intensity of 140–180 μmol · m−2 · s−1. Under these conditions, the asexual life cycle of Astrephomene was completed in approximately 24 h, and the culture was highly synchronized with the light–dark cycle. Almost all (99 %) colonies of Astrephomene initiated embryogenesis 6 h before the onset of the dark period. In Eudorina, the asexual life cycle was completed in approximately 48 h, and the culture was not highly synchronized with the light–dark cycle. Embryogenesis was initiated 1 or 2 h after the onset of the dark period in 30 % of the Eudorina colonies.
Light microscopy time-lapse imaging
Embryogenesis of Astrephomene gubernaculifera 2014-1002-YkAs8 and Eudorina sp. 2010-623-F1-E8 was observed by time-lapse light microscopy based on a method reported previously  with some modifications (Additional file 5: Figure S3). To examine the embryos from anterior–lateral, lateral, posterior–lateral and posterior angles, fully mature vegetative colonies of Astrephomene were fragmented into several parts using a Dounce tissue grinder (Wheaton Industries Inc., Millville, NJ, USA) and attached to coverslips coated with polyethylenimine. Then, the coverslips were placed on slides and sealed with Vaseline. Preparations were observed using a BX-53 microscope (Olympus, Tokyo, Japan) equipped with Nomarski interference optics. Plural photomicrographs with different optical sections were obtained using DP Controller 1. 2. 1108 (Olympus) at 1-min intervals with manual successive changes in focus. In Eudorina, as fragmentation of the colonies was not possible, fully mature colonies were directly attached to coverslips and observed as described above. Only anterior–lateral view images were obtained from Eudorina. The images of both species were analyzed and processed using ImageJ 1.50b (National Institutes of Health, Bethesda, MD, USA).
Indirect immunofluorescence microscopy
To verify the behavior of daughter protoplasts during the embryogenesis of Astrephomene gubernaculifera 2014-1002-YkAs8 and Eudorina sp. 2010-623-F1-E8, immunostaining of basal bodies and nuclei was performed as described previously , with the exception of the primary and secondary antibodies used to stain nuclei. A mouse monoclonal anti-histone H1 antibody (clone F152.C25.WJJ, Merck Millipore Corp., Darmstadt, Germany) diluted 1:500 with blocking buffer (0.44 % gelatin [Sigma-Aldrich, St. Louis, MO, USA], 0.05 % NaN3, 1 % BSA [Sigma-Aldrich], 0.1 % Tween 20 [Sigma-Aldrich] in phosphate-buffered saline) was used as the primary antibody to stain nuclei. The secondary antibody was an Alexa Fluor 568-conjugated goat anti-mouse IgG (Invitrogen, Carlsbad, CA, USA) diluted 1:500 with blocking buffer. Because a rabbit anti-CrSAS-6 antibody  and Alexa Fluor 488 goat anti-rabbit IgG (Invitrogen) were used as the primary and secondary antibodies, respectively, for staining basal bodies, the double staining of basal bodies and nuclei was feasible. Preparations were observed using a BX-60 microscope (Olympus) equipped with Nomarski interference optics, a mercury lamp, and filter sets with DP Controller 1.2.1108 (Olympus). Differential interference contrast images and fluorescence images were merged using Adobe Photoshop CC (Adobe Systems Inc., San Jose, CA, USA). To evaluate the specificity of the anti-CrSAS-6 antibody for Astrephomene and Eudorina, western blot analysis was carried out as described previously  (Additional file 5: Figure S4).
We would like to thank Dr. Osami Misumi and Ms. Mizuho Sugasawa for providing soil samples to establish new strains of Astrephomene.
This work was supported by Grants-in-Aid for Scientific Research (A) (grant number 16H02518 to HN), and Scientific Research (B) (grant number 25304012 to HN) from MEXT/JSPS KAKENHI.
Availability of data and materials
The new rbcL sequence has been deposited to DDBJ/EMBL/GenBank (accession number: LC188997). The sequence alignment used for molecular identification of the new strain of Astrephomene (Additional file 5: Figure S2) has been deposited in TreeBASE (https://treebase.org/treebase-web/home.html; study ID: 19979). Astrephomene gubernaculifera strain 2014-1002-YkAs8 and Eudorina sp. strain 2010-623-F1-E8 are available from Microbial Culture Collection at the Institute for National Environmental Studies (http://mcc.nies.go.jp/localeAction.do?lang=en) as NIES-4017 and NIES-4018, respectively. All the other data generated or analysed during this study are included in this published article and its Additional files 1, 2, 3, 4, 5 and 6.
SY, YA, HKT and HN conceived and designed the experiments. AN and MH contributed the reagent. SY performed the experiments and analyzed the data. SY and HN wrote the manuscript. All authors read and approved the manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Michod RE. On the transfer of fitness from the cell to the multicellular organism. Biol Philos. 2005;20:967–87.View ArticleGoogle Scholar
- Grosberg RK, Strathmann RR. The evolution of multicellularity: a minor major transition? Annu Rev Ecol Evol Syst. 2007;38:621–54.View ArticleGoogle Scholar
- Kirk DL. A twelve-step program for evolving multicellularity and a division of labor. Bioessays. 2005;27:299–310.View ArticlePubMedGoogle Scholar
- Nozaki H, Misawa K, Kajita T, Kato M, Nohara S, Watanabe MM. Origin and evolution of the colonial Volvocales (Chlorophyceae) as inferred from multiple, chloroplast gene sequences. Mol Phylogenet Evol. 2000;17:256–68.View ArticlePubMedGoogle Scholar
- Herron MD, Michod RE. Evolution of complexity in the volvocine algae: transitions in individuality through Darwin’s eye. Evolution. 2008;62:436–51.View ArticlePubMedGoogle Scholar
- Herron MD, Hackett JD, Aylward FO, Michod RE. Triassic origin and early radiation of multicellular volvocine algae. Proc Natl Acad Sci U S A. 2009;106:3254–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Merchant SS, Prochnik SE, Vallon O, Harris EH, Karpowicz SJ, Witman GB, et al. The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science. 2007;318:245–51.View ArticlePubMedPubMed CentralGoogle Scholar
- Prochnik SE, Umen J, Nedelcu AM, Hallmann A, Miller SM, et al. Genomic analysis of organismal complexity in the multicellular green alga Volvox carteri. Science. 2010;329:223–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Arakaki Y, Kawai-Toyooka H, Hamamura Y, Higashiyama T, Noga A, et al. The simplest integrated multicellular organism unveiled. PLoS One. 2013;8:e81641.View ArticlePubMedPubMed CentralGoogle Scholar
- Hanschen ER, Marriage TN, Ferris PJ, Hamaji T, Toyoda A, et al. The Gonium pectorale genome demonstrates co-option of cell cycle regulation during the evolution of multicellularity. Nat Commun. 2016;7:11370.View ArticlePubMedPubMed CentralGoogle Scholar
- Nozaki H, Itoh M. Phylogenetic relationships within the colonial Volvocales (Chlorophyta) inferred from cladistic analysis based on morphological data. J Phycol. 1994;30:353–65.View ArticleGoogle Scholar
- Herron MD, Desnitskiy AG, Michod RE. Evolution of developmental programs in Volvox (Chlorophyta). J Phycol. 2010;46:316–24.View ArticleGoogle Scholar
- Viamontes GL, Kirk DL. Cell shape changes and the mechanism of inversion in Volvox. J Cell Biol. 1977;75:719–30.View ArticlePubMedGoogle Scholar
- Viamontes GL, Fochtmann LJ, Kirk DL. Morphogenesis in Volvox: analysis of critical variables. Cell. 1979;17:537–50.View ArticlePubMedGoogle Scholar
- Green KJ, Kirk DL. Cleavage patterns, cell lineages, and development of a cytoplasmic bridge system in Volvox embryos. J Cell Biol. 1981;91:743–55.View ArticlePubMedGoogle Scholar
- Nishii I, Ogihara S. Actomyosin contraction of the posterior hemisphere is required for inversion of the Volvox embryo. Development. 1999;126:2117–27.PubMedGoogle Scholar
- Nishii I, Ogihara S, Kirk DL. A kinesin, InvA, plays an essential role in Volvox morphogenesis. Cell. 2003;113:743–53.View ArticlePubMedGoogle Scholar
- Fulton AB. Colonial development in Pandorina morum II. colony morphogenesis and formation of the extracellular matrix. Dev Biol. 1978;64:236–51.View ArticlePubMedGoogle Scholar
- Gottlieb B, Goldstein ME. Colony development in Eudorina elegans (Chlorophyta, Volvocales). J Phycol. 1977;13:358–64.Google Scholar
- Marchant HJ. Colony formation and inversion in the green alga Eudorina elegans. Protoplasma. 1977;93:325–39.View ArticleGoogle Scholar
- Gerisch G. Die Zelldifferenzierung bei Pleodorina californica Shaw und die Organisation der Phytomonadinenkolonien. Arch Protistenkd. 1959;104:292–358.Google Scholar
- Kikuchi K. Cellular differentiation in Pleodorina californica. Cytologia. 1978;43:153–60.View ArticleGoogle Scholar
- Hallmann A. Morphogenesis in the family Volvocaceae: different tactics for turning an embryo right-side out. Protist. 2006;157:445–61.View ArticlePubMedGoogle Scholar
- Pocock MA. Two multicellular motile green algae, Volvulina Playfair and Astrephomene, a new genus. Trans Roy Soc S Afr. 1953;34:103–27.View ArticleGoogle Scholar
- Nozaki H. Morphology and taxonomy of two species of Astrephomene (Chlorophyta, Volvocales) in Japan. J Jpn Bot. 1983;58:345–52.Google Scholar
- Stein JR. A morphological study of Astrephomene gubernaculifera and Volvulina steinii. Am J Bot. 1958;45:388–97.View ArticleGoogle Scholar
- Nakazawa Y, Hiraki M, Kamiya R, Hirono M. SAS-6 is a cartwheel protein that establishes the 9-fold symmetry of the centriole. Curr Biol. 2007;17:2169–74.View ArticlePubMedGoogle Scholar
- Hoops HJ, Floyd GL. Mitosis, cytokinesis and colony formation in the colonial green alga Astrephomene gubernaculifera. Br Phycol J. 1982;17:297–310.View ArticleGoogle Scholar
- Pringsheim EG. Pure Cultures of Algae. London: Cambridge University Press; 1946.Google Scholar
- Nozaki H, Kuroiwa H, Mita T, Kuroiwa T. Pleodorina japonica sp. nov. (Volvocales, Chlorophyta) with bacteria-like endosymbionts. Phycologia. 1989;28:252–67.View ArticleGoogle Scholar
- Yamada TK, Tsuchikane Y, Wu J, Sekimoto H, Miyaji K, Nozaki H. Morphology and molecular phylogeny of Eudorina sp. (Volvocaceae, Chlorophyceae) from Taiwan. Hikobia. 2008;15:135–43.Google Scholar
- Hiraide R, Kawai-Toyooka H, Hamaji T, Matsuzaki R, Kawafune K, Abe J, et al. The evolution of male–female sexual dimorphism predates the gender-based divergence of the mating locus gene MAT3/RB. Mol Biol Evol. 2013;30:1038–40.View ArticlePubMedPubMed CentralGoogle Scholar