Fig. 3

Drosocin in the subgenus Drosophila. a The subgenus Drosophila drosocin has a strongly conserved ERPPY motif at the proline-rich N-terminus, followed by the drosocin domain PRPT, which includes a critical threonine residue. This domain is followed by furin-like cleavage sites (annotated as “Furin”). The presence of both furin-like cleavage sites and the key threonine residue indicate that this transcript is likely processed to produce multiple copies of a mature drosocin peptide glycosylated at its PRPT threonine. This alignment presents the signal peptide and first drosocin repeat in each species, and does not include tandem drosocin repeats, which vary in number and sequence depending on species. b Drosocin in the subgenus Drosophila is found within the gene region of DSCAM1 and Gr43a, ~7.36 million base pairs displaced from the drosocin gene region in the subgenus Sophophora. The D. virilis Gr43A gene region is included here