Skip to main content
Fig. 2 | BMC Evolutionary Biology

Fig. 2

From: Development of a toolbox to dissect host-endosymbiont interactions and protein trafficking in the trypanosomatid Angomonas deanei

Fig. 2

Sequence analysis confirms insertion of resistance cassette in the γ-amastin locus. Genomic DNA extracted from Δ-γ-amaHyg SKO clone 1 (see Additional file 2: Figure S3a) was used as a template for PCR with primers binding to the gDNA outside of the cassette and inside the resistance markers. The PCR products obtained were sequenced. The alignment shows: Clone1-5′ or 3′ UTR, sections of the genomic sequence of the transformant resulting from HR between the gDNA and the resistance cassette (pAdea γ-Ama/Hyg); clone1-5′UTR/Hyg and clone1-Hyg/3′UTR, sections of the genomic sequence of the transformant surrounding the start and stop codon of the hyg gene, respectively; γ-Ama/Hyg rScaffold, the expected genomic sequence organization of the recombinant Δ-γ-AmaHyg locus; γ-Ama WT Scaffold, wild-type sequence at the γ-amastin locus. The map underneath the alignment indicates primer binding sites (arrows) and sizes of resulting PCR products. Red arrowheads indicate the position at which the cassette inserted by HR into the genome and black arrowheads denote the start and stop codon of the hyg and γ-amastin ORFs. The striped rectangles represent genomic regions upstream and downstream of the insertion sites for the cassettes. Light blue rectangles represent the 5′- and 3′-flanking regions (FR) of the γ-amastin gene that are present in the cassette. Yellow rectangles represent hyg

Back to article page