Fig. 5

Heterologous expression and subcellular localization of EGFP in A. deanei. (a) The pAEX series of plasmids. Violet rectangles, δ-amastin 5′- and 3′-flanking regions (δ-ama FRs); blue rectangle, glyceraldehyde 3-phosphate dehydrogenase I-II intergenic region (GAPDH IR); magenta arrow, neo; green arrow, enhanced green fluorescence protein (EGFP); red highlights, position of the T. brucei dihydrolipoyl dehydrogenase mTP (mito) and the peroxisomal targeting sequence type 1 (PTS1). (b) Cells transfected with pAEX cassettes encoding the EGFP, mito-EGFP containing an N-terminal mTP, or EGFP-SKL containing the C-terminal PTS1 were spread on polylysine-coated glass slides, fixed in 2 % v/v ice-cold solution of paraformaldehyde in PBS, and stained with DAPI for 5 min before visualization. Scale bar: 2 μm