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Fig. 2 | BMC Evolutionary Biology

Fig. 2

From: Assessing segmental versus non-segmental features in the ventral nervous system of onychophorans (velvet worms)

Fig. 2

Organisation of the ventral nervous system in Onychophora as revealed by cross sections. ad Maximum projections confocal micrographs of Vibratome sections of a specimen of Euperipatoides rowelli double-labelled with anti-acetylated α-tubulin serum (glow mode) and DNA marker Hoechst (bisbenzimide; blue). Dorsal is up in all images. Median is left (bd). a Cross section through a whole specimen showing position of the ventral nerve cords that are connected via a median commissure (mc). bd Cross section of a nerve cord. b Anti-acetylated α-tubulin labelling showing the neuropil. c DNA labelling with Hoechst showing the perikaryal layer. d Merged image of the same specimen as in (b) and (c). gt, gut; hn, heart nerve; ln, leg nerve; mc, median commissure; nc, nerve cord; np, neuropil; pe, perikaryal layer; rc, ring commissure. Scale bars: 100 μm (a), 50 μm (bd)

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