Fig. 2From: Assessing segmental versus non-segmental features in the ventral nervous system of onychophorans (velvet worms)Organisation of the ventral nervous system in Onychophora as revealed by cross sections. a–d Maximum projections confocal micrographs of Vibratome sections of a specimen of Euperipatoides rowelli double-labelled with anti-acetylated α-tubulin serum (glow mode) and DNA marker Hoechst (bisbenzimide; blue). Dorsal is up in all images. Median is left (b–d). a Cross section through a whole specimen showing position of the ventral nerve cords that are connected via a median commissure (mc). b–d Cross section of a nerve cord. b Anti-acetylated α-tubulin labelling showing the neuropil. c DNA labelling with Hoechst showing the perikaryal layer. d Merged image of the same specimen as in (b) and (c). gt, gut; hn, heart nerve; ln, leg nerve; mc, median commissure; nc, nerve cord; np, neuropil; pe, perikaryal layer; rc, ring commissure. Scale bars: 100 μm (a), 50 μm (b–d)Back to article page