Fig. 5

Spatial changes of amphioxus CYP26 expression in response to retinoic acid (RA) signaling alterations. Whole mount in situ hybridization experiments were carried out for CYP26-1 (a-f), CYP26-2 (g-m), and CYP26-3 (n-s) on amphioxus (Branchiostoma lanceolatum) embryos treated at the late blastula stage with either DMSO (as control), all-trans RA (1 μM) or RAR antagonist BMS009 (1 μM). The BMS009-treated embryo in (c) and larva in (f) were subjected to an extended coloration period. Mid neurula embryos at 20 h of development (a-c, g-j, n-p) and early larvae at 48 h of development (d-f, k-m, q-s) are shown as lateral views with anterior to the left and the dorsal side up. Scale bars are 100 μm