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Fig. 6 | BMC Evolutionary Biology

Fig. 6

From: Lineage-specific duplication of amphioxus retinoic acid degrading enzymes (CYP26) resulted in sub-functionalization of patterning and homeostatic roles

Fig. 6

Dynamics of amphioxus CYP26 gene expression following protein synthesis inhibition and retinoid treatments. Expression of CYP26-1, CYP26-2, and CYP26-3 was assessed in amphioxus (Branchiostoma lanceolatum) by quantitative real time PCR (qPCR) at the mid neurula stage (at 20 h of development) following protein synthesis inhibition by puromycin treatment (200 μg/ml) at 19 h of development and subsequent treatment with DMSO (as control), all-trans RA (1 μM) or RAR antagonist BMS009 (1 μM) for 1 h. Relative normalized expression (left panel) and fold change of expression levels (right panel) relative to the controls are shown

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