Fig. 2

Deletion detection in the eGFP gene. Agarose gels with RT-PCR products of the region encompassing the eGFP gene. The TEV and TEV-eGFP are shown for comparative purposes. The negative controls are healthy plants and PCR controls (C-). a TEV-eGFP in D. stramonium has 10 independent lineages (L1-L10). A deletion encompassing the eGFP gene was detected in one lineage (L8) in the second 9-week passage. This deletion went to a high frequency in the subsequent passage. b TEV-eGFP in N. benthamiana has six independent lineages (L1-L6). A deletion bigger than the size of eGFP was detected in one lineage (L4) in the fourth 6-week passage. This deletion was not fixed in the two subsequent passages. A small deletion was detected in the fifth and sixth 6-week passage in L1