Fig. 4

Virus accumulation of the evolved and ancestral lineages. Virus accumulation, as determined by accumulation experiments and RT-qPCR at 10 dpi, of TEV, the ancestral TEV-eGFP, and the evolved TEV-eGFP lineages in N. benthamiana (a) and D. stramonium (b). TEV and the evolved lineage with a deletion in the eGFP gene (B; L8) are indicated with the orange bars. The ancestral TEV-eGFP and the evolved lineages with an intact eGFP gene are indicated with the green bars. No significant differences between TEV and TEV-eGFP ancestral were found, nor between these and the evolved lineages (t-test with Holm-Bonferroni correction for multiple tests). Error bars represent SD of the plant replicates