Fig. 2

Evolutionary analysis of G6Pase genes. a G6PC2 is shown with its predicted membrane topology; protein regions are coloured in hues of blue according to the percentage of negatively selected sites (FEL p value < 0.1). Positively selected sites in the mammalian phylogeny (black) and in Homininae (blue) are reported on the structure. Missense variants associated with FG are shown in red. Asterisks denote negatively selected sites. The glycosylation site is also shown. b Violin plots of selection coefficients (median, white dot; interquartile range, black bar) for the three G6Pase genes. Selection coefficients (γ) are classified as strongly beneficial (100, 50), moderately beneficial (10, 5), weakly beneficial (1), neutral (0), weakly deleterious (−1), moderately deleterious (−5, −10), strongly deleterious (−50, −100), and inviable (−500). c Phylogenetic tree for primate G6PC3 genes. Branches are color-coded according to RELAX results: blue, significant evidence of relaxed selection; orange, no significant evidence of relaxation