- Research article
- Open Access
Very high MHC Class IIB diversity without spatial differentiation in the mediterranean population of greater Flamingos
© The Author(s). 2017
- Received: 29 September 2016
- Accepted: 6 February 2017
- Published: 20 February 2017
Selective pressure from pathogens is thought to shape the allelic diversity of major histocompatibility complex (MHC) genes in vertebrates. In particular, both local adaptation to pathogens and gene flow are thought to explain a large part of the intraspecific variation observed in MHC allelic diversity. To date, however, evidence that adaptation to locally prevalent pathogens maintains MHC variation is limited to species with limited dispersal and, hence, reduced gene flow. On the one hand high gene flow can disrupt local adaptation in species with high dispersal rates, on the other hand such species are much more likely to experience spatial variation in pathogen pressure, suggesting that there may be intense pathogen mediated selection pressure operating across breeding sites in panmictic species. Such pathogen mediated selection pressure operating across breeding sites should therefore be sufficient to maintain high MHC diversity in high dispersing species in the absence of local adaptation mechanisms. We used the Greater Flamingo, Phoenicopterus roseus, a long-lived colonial bird showing a homogeneous genetic structure of neutral markers at the scale of the Mediterranean region, to test the prediction that higher MHC allelic diversity with no population structure should occur in large panmictic populations of long-distance dispersing birds than in other resident species.
We assessed the level of allelic diversity at the MHC Class IIB exon 2 from 116 individuals born in four different breeding colonies of Greater Flamingo in the Mediterranean region. We found one of the highest allelic diversity (109 alleles, 2 loci) of any non-passerine avian species investigated so far relative to the number of individuals and loci genotyped. There was no evidence of population structure between the four major Mediterranean breeding colonies.
Our results suggest that local adaptation at MHC Class IIB in Greater Flamingos is constrained by high gene flow and high MHC diversity appears to be maintained by population wide pathogen-mediated selection rather than local pathogen-mediated selection. Further understanding of how pathogens vary across space and time will be crucial to further elucidate the mechanisms maintaining MHC diversity in species with large panmictic populations and high dispersal rates.
- MHC genes
- Allelic diversity
- Pathogen-mediated balancing selection
- Local adaptation
- Greater flamingos
Genetic diversity is an essential component of the adaptive potential of populations [1, 2]. The major histocompatibility (MHC) genes code for glycoproteins which present antigens to T-cells playing an integral role in the adaptive immune response . The MHC genes form a multigene family, and are known to be among the most polymorphic genes of the vertebrate genome, with most populations exhibiting a high level of MHC allelic diversity in terms of both allele number and sequence divergence [4, 5]. This multigene family has therefore become an important model of adaptive variation in natural populations of vertebrates [4, 5].
The maintenance of MHC polymorphism is generally attributed to powerful pathogen-mediated balancing selection, with gene conversion and recombination, maternal-foetal interactions and sexual selection also thought to play a role [4, 5]. Since selection from local pathogens may be sufficiently strong to change MHC allele frequencies within the short evolutionary time of a few generations [6–8], we can expect local pathogen-mediated selection at the MHC to be stronger than either neutral forces (such as gene flow and genetic drift) or pathogen-mediated selection at the scale of the metapopulation. Such local pathogen-mediated selection can occur if there is spatial and temporal variation in pathogen-mediated selection (“fluctuating selection”)  and/or local cyclical selection between host and pathogen (“negative frequency-dependent selection”) [10–12], and both mechanisms are thought to play a crucial role in maintaining MHC diversity. Indeed, stronger population structure at the MHC than at neutral markers have been demonstrated in numerous species that show some level of population structure at neutral markers (reviewed in ). However, the occurrence of local adaptation at the MHC in species with high gene flow remains to be investigated.
Long-lived and dispersing species with large geographical distributions, often associated with panmixia at neutral markers, face a particular challenge: individuals are likely to encounter during their life heterogeneous environmental conditions, each associated with distinct pathogen communities . Indeed, species that are migratory [14, 15] and have large geographical distributions [14, 16, 17] are known to have more diverse parasitic communities. As a consequence of encountering more diverse parasitic communities, migratory or long-distance dispersing species may be under more intense pathogen mediated selection than resident species. In support of the latter, a recent comparative study has found that the rate of non-synonymous nucleotide substitution across the antigen binding regions of the MHC class IIB (a signal of balancing selection) is significantly higher in migratory species . The latter study also found that colonial species had a higher rate of non-synonymous nucleotide substitution, which suggests that the high contact between conspecifics in colonial species leads to elevated transmission rates and therefore increased balancing selection of MHC genes . However, the high gene flow of high dispersing species is likely to disrupt local adaptation and other pathogen-mediated balancing selection mechanisms are likely to be maintaining MHC diversity. For instance, frequency-dependent selection may be operating across breeding sites in panmictic species. Another non-mutually exclusive hypothesis, which could also be operating across breeding sites in panmictic species, is the “overdominant heterozygote advantage” hypothesis. The latter proposes that MHC heterozygotes will be fitter than the fittest MHC homozygote because they will be able to recognize a greater diversity of parasites [19, 20]. Higher MHC allelic diversity can thus be expected in colonial and long-distance dispersing species with little or no spatial differentiation between breeding sites than locally adapted resident species, based on the idea that higher pathogen-mediated balancing selection is operating across breeding sites in panmictic species. In addition, such long-range dispersing species should have no MHC population structure since gene flow prevents local adaptation.
Summary statistics of MHC Class IIB exon 2 variation for the four breeding colonies of Greater Flamingos
Year of sampling
Mean # alleles per individual
Mean AAdist (±SE)
Mean AAdistSelect (±SE)
2008 & 2009
2007 & 2009
MHC Class IIB exon2 genotyping
Details of MHC Class IIB exon 2 genotyping can be found in Gillingham et al.  which genotyped the individuals used in this study using barcoded PCRs in 454 Titanium pyrosequencing run. Briefly, 163–165 bp of the 267–270 bp of the MHC Class IIB exon 2 was amplified which code for nine positively selected codon sites . Assignment of barcoded sequences to individual barcodes and allele validation was carried out with the aid of SEquence Sorter & AMplicon Explorer (SESAME) . We used a slightly modified version of Sommer et al.’s  pipeline and allele validation followed the following rules: 1) all reads whose length suggested a shift in reading frame during sequencing were discarded; 2) all variants that were represented only once in the run (i.e. singletons) were assumed to be artefacts; 3) we assumed that artefactual variants should occur at lower frequencies than true variants and we used the conservative T 2 threshold recommended by Galan et al.  with F ij of 4% or above to assign reads as alleles; 4) all sequences that had a 1–2 base pair difference to one of the validated alleles within the sample were assumed to be artefacts; 5) all sequences that had more than a 2 base pair difference from validated alleles and were also validated as alleles if they were classified as alleles in other individuals; 6) the remaining sequences that had more than a 2 base pair difference from a validated allele were validated as alleles only if their frequency exceeded any artefact; 7) we assumed that chimeras were always present together with the true alleles from which they were formed and in lower frequency. Accordingly chimeras were easily identified when reads were aligned within individuals in SESAME and were discarded if in lower frequency than source validated alleles. Gillingham et al.  reported a maximum of 4 alleles per individual suggesting two loci for this marker . Of the 126 individuals genotyped, we used in subsequent analyses the 116 for which the authors achieved a 99.9% confidence in allele calling (>300 reads per individual) (see Gillingham et al.  for details of allele calling and simulations). Based on 10 duplicates and 2 triplicates Gillingham et al.  found a repeatability of 100% in allele calling.
Population differentiation analyses
First, we estimated nucleotide diversity and number of segregating sites using DnaSP version 5 . Second, we measured MHC population differentiation. There are many measures of population differentiation and ways of partitioning genetic variance, with constant debate and research among population geneticists about which measure is the best to use in which circumstance [29–33]. Multilocus genes such as the MHC add the additional complication that their complex nature often violates assumptions required to apply several of these methods (e.g. Hardy-Weinberg equilibrium, linkage disequilibrium, mixed inheritance patterns). A further complication arises when alleles cannot be assigned to loci, as is often the case for MHC datasets in non-model organisms, preventing the identification of heterozygote and homozygote genotypes. As a result, allele frequencies cannot be accurately estimated as the frequency of common alleles is underestimated and that of rare alleles overestimated. We therefore chose to rely on the metric Rho (also known as p statistic) devised by Ronfort et al.  for genotype data with multiple alleles per locus in each individual as found in polyploidy species. The Rho statistic is independent of both the ploidy level and the amount of within-individual diversity. This statistic has also recently been demonstrated by simulation to be less biased when assumptions of diploid inheritance are violated than Nei’s GST , G″ST  and Jost’s D [29, 32]. We estimated the Rho statistic in conjunction with traditional Fst  to measure MHC population differentiation using the SPAGeDi software . We carried out this analysis on individual MHC Class IIB exon 2 alleles based on the full length nucleotide sequence, allelic lineages based on codon sites which were shown to be under positive selection by Gillingham et al.  (i.e. different sequences were considered the same allele if they did not differ in positively selected codon sites) and allelic lineages based on the full length translated amino acid sequences.
where, X is the number of nearest neighbour belonging to the same geographical locality divided by the total number of nearest neighbour for sequence j. Hence, if X j is equal to 1, then all nearest sequences belonged to individuals sampled within the same geographical locality; and, conversely if X j is equal to 0, all nearest sequences were from a different geographical locality. In a pairwise comparison between populations, a S nn that is significantly greater than 0.5 therefore indicates evidence of some population structure, as does a global S nn which is significantly greater than 0.25 when simultaneously comparing four populations. The S nn statistic was estimated using the PopGenome package  in R and significant departure from predicted values given panmixia were estimated by permutation using 1000 reshuffling in R . We carried out this analysis on both full sequences and on sequences of codon sites which were shown to be under positive selection by Gillingham et al. .
where for population k, N is the number of pairwise comparisons, D ab is the number of alleles not shared between individual a and individual b, A a and A b are the total number of unique alleles in individuals a and b. APD therefore measures within-population genetic variation. We tested whether between breeding colonies APD was significantly different from within breeding colonies APD by permutation using 100,000 reshuffling. APD calculations were performed using R .
Finally, we estimated MHC heterozygosity with three metrics that did not require the assignment of MHC alleles to loci (since this is not possible in our dataset ): (i) the number of MHC alleles per individual; (ii) the mean amino acid allele divergence within an individual over the entire translated sequences (of the 163–165 bp exon 2 nucleotide sequences; AAdist), and (iii) the mean amino acid allele divergence within an individual over sites found to be under positive selection by Gillingham et al.  (AAdistSelect). We obtained the proportion of base pair differences between sequences required to compute AAdist and AAdistSelect using MEGA 7 . We tested differences in the number of MHC alleles per individual between breeding colonies using a generalized linear model (GLM) with a Poisson distribution and a log link. For testing differences in AAdist and AAdistSelect between breeding colonies we used a GLM model with a binomial distribution (since AAdist and AAdistSelect are averages of proportional data) and a logit link function. All GLMs were fitted in R .
Microsatellite genotyping and heterozygosity correlation with MHC Class IIB exon 2
The 116 samples used for Greater Flamingo MHC Class IIB exon 2 differentiation in this study were previously selected for microsatellite genotyping by Geraci et al. for 13 loci: PrA2, PrD3, PrD4, PrD5, PrD7, PrD9, PrA102, PrA110, PrA113, PrC109, PrD108, PrD121 and PrD126. Nine individuals were excluded from this analysis because fewer than 8 microsatellite loci were successfully genotyped for these individuals. In order to compare MHC differentiation to differentiation at neutral markers we estimated F ST  for microsatellite data differentiation as calculated by the SPAGeDi software .
Comparison with other avian non-passerine species of MHC Class IIB exon 2 allelic diversity
Number of alleles for MHC Class IIB exon2 relative to the number of loci and individuals genotyped in non-passerine birds reported in the literature with 100 or more individuals genotyped, showing species name (common name and Latin name), number of individuals genotyped in the study, maximum number of loci based on the maximum number alleles found within an individual in the population, number of alleles found in the study, the ratio between number of alleles and number of individuals, the ratio between number of alleles, number of individuals and number of loci, whether the species is migratory and/or long distance disperser and the citation of the study
Species (Common name)
Species (Latin name)
# of ind.
# of loci
# of alleles
# of alleles /# of ind.
# of alleles /# of ind./# of loci
Alcaide et al., 2008 
Knafler et al., 2012 
Alcaide et al., 2014 
Ekblom et al., 2007 
Greater Prairie Chicken
Minias et al., 2016 
Leach's Storm Petrel
Dearborn et al., 2016 
Lei et al., 2016 
Promerová et al., 2013 
Blakiston’s Fish Owl
Kohyama et al., 2015 
Meyer-Lucht et al., 2016 
Attwater’s Prairie Chicken
Bateson et al., 2016 
As reported in Gillingham et al. , we detected a very high diversity of MHC Class IIB exon 2 with 109 alleles at two loci among 116 individuals (Table 2). The mean number of alleles per individual was 3.44 (Table 1), and 63 individuals had 4 alleles (54%), 42 individuals had 3 (36%), 10 individuals had 2 (9%), and 1 individual had 1 (1%). The number of alleles identified per population was between 55 and 63 identified alleles (Table 1). Out of 109 alleles identified from nucleotide sequences across the four breeding sites, 39 were private alleles (Table 1), however all of these alleles were rare and only present within either a single individual (34 alleles) or two individuals (5 alleles). The 109 alleles from nucleotides sequences translate into 104 different amino acid sequences. When comparing with other non-passerine avian species, allelic diversity was higher than all species that had been sampled more than greater flamingos (n > 116) except for the Eurasian coot (Table 2). When rarefying coot sample size to 116 (resampling 10000 times with no replacement) median number of alleles was 130 (95% confidence intervals was 119–142). When rarefying the flamingo dataset (resampling 10000 times with no replacement) to the sample of the two species in Table 2 with a smaller dataset, greater flamingo allelic diversity remained much greater than those species (n = 100, median number of alleles = 104, 95% confidence intervals = 100–107; n = 108, median number of alleles = 107, 95% confidence intervals = 104–109).
Pairwise Rho statistic  of MHC Class IIB Exon 2 for alleles called from full nucleotide sequences (below diagonal) and alleles called from nucleotide sequences of sites under positive section (above diagonal) (a); pairwise F ST statistic  of MHC Class IIB Exon 2 for alleles called from full nucleotide sequences (below diagonal) and alleles called from nucleotide sequences of sites under positive section (above diagonal) (b); pairwise S nn statistic  of MHC Class IIB Exon 2 for full length nucleotide sequences (below diagonal) and for nucleotide sequences of sites under positive section (above diagonal) (b) and, pairwise F ST values for 13 microsatellite loci (above diagonal) for four breeding colonies of Greater Flamingos across the Mediterranean basin
a. Rho statistic for MHC Class IIB exon 2
b. F ST statistic for MHC Class IIB exon 2
c. S nn statistic for MHC Class IIB exon 2
d. F ST statistic for 13 microsatellite locus
Further supporting no population structure at the MHC Class IIB exon 2, global S nn did not significantly deviate from the predicted value for a panmictic population of 0.25 when analysing full length sequences (S nn = 0.239; p = 0.714) and sequences known to be under positive selection (S nn = 0.246; p = 0.608). Similarly pairwise comparison between breeding colonies never significantly deviated from the predicted panmictic value of 0.5 (Table 3c). Average percentage difference (APD) was very high suggesting very high within-population MHC variation (Table 1). Furthermore, APD within colony was not significantly different from APD between breeding colonies (p = 0.590) and a similar result was found when analysing APD from amino acid sequences (p = 0.493). The mean number of alleles per individual (χ 2 = 0.132; df = 3; p = 0.988), mean AAdist (χ 2 = 3.069; df = 3; p = 0.381) and mean AAdistSelect (χ 2 = 0.449; df = 3; p = 0.930) were not significantly different between breeding colonies.
To the best of our knowledge, the MHC allelic diversity in greater flamingos is one of the highest reported so far (this study & ) relative to the number of individuals genotyped in non-passerine birds, despite the fact that they appear to harbour only two MHC Class IIB loci (Table 2). Only Coots have slightly higher MHC allelic diversity than Greater flamingos, with 130 alleles (95% confidence intervals was 119–142) compared to the Flamingo’s 109 when rarefied to the same sample size, although Coots harbour three loci. Furthermore, the fact that allele calling is based on 163–165 bp of the 267–270 bp exon 2 sequence suggests that we may be slightly underestimating allelic diversity since alleles may differ in regions outside the amplified segment. The variation we found in the number of alleles per individual can be explained by either variation in loci copy number as reported in certain bird species e.g. [51, 52] or balancing selection maintaining identical alleles across duplicated loci e.g. . Alternatively, we cannot exclude that our design of primers was not optimal and may have led to some allele dropout. Indeed designing optimal primers without any allele dropout is notoriously difficult to achieve in non-model organisms . However the latter is unlikely to bias population differentiation analyses since the same primers were used across all individuals and populations.
Passerines frequently appear to also present a very high diversity of MHC alleles [46–50] but this generally seems to be due to their high number of MHC gene copies . Furthermore due to the high number of pseudogenes reported in passerines, unbiased functional allelic diversity of these species is unclear and difficult to quantify. Two other examples of non-passerine bird species with a high allelic MHC diversity are the Eurasian Coot, Fulica atra,  (265 alleles; 906 individuals; 3 loci) and the Lesser Kestrel Falco naumanni  (103 alleles; 121 individuals; 1 loci). Like Greater Flamingos, the Eurasian Coot has a large geographical distribution and form large populations , whilst the colonial Lesser Kestrel is a long distance migratory species and forms small populations . One possibility is that high gene flow across breeding sites in the Mediterranean population of Greater Flamingos is relatively recent and that range-wide MHC diversity arose through local adaptation. However, we believe the latter to be highly unlikely for the following reason. Geraci et al.  have shown that flamingo populations have undergone a bottleneck followed by rapid growth and expansion, with the average time since expansion being estimated to be 696 421 yr (90% CI: 526 316–1 131 579 yr), suggesting that high gene flow in this species is not recent. A possible increase in gene flow remains possible since over the last three centuries wetlands and favourable nesting places for flamingos have become scarce . However, there is no reason to believe that the species was less nomadic in the ancient past than today. Our study therefore supports that in Greater Flamingos, although high dispersal rates disrupts the local adaptation mechanisms that are predicted to maintain MHC diversity, pathogen-mediated balancing selection at the scale of the large and panmictic Mediterranean population is sufficiently strong to generate high MHC allelic diversity. However, a greater characterisation of MHC diversity of migratory and non-migratory bird species than is currently available in the literature, followed by a meta-analysis, would be required to formally test whether ecology and life history traits of species (such as migration, long distance dispersal, wide geographic distribution and colonial breeding) predicts population wide MHC allelic diversity.
Theory predicts that local adaptation and divergent selection between populations should be favoured when gene flow is at an intermediate level . Such intermediate levels of gene flow may occur between breeding sites of colonial breeding species if philopatric behaviour is coupled with regular dispersal . In lesser kestrels, pathogen-mediated balancing selection at the local scale of the population appears to be maintaining high allelic diversity (stronger local population structure at MHC markers than at neutral makers) . A similar result was reported in the migratory but philopatric loggerhead sea turtles (Caretta caretta) which showed distinct MHC differentiation between breeding colonies, despite close proximity between sites (80–260 km) . However, evidence that local adaptation can occur despite high gene flow has only been reported in a few studies of marine fish [59–62]. It is also worth noting that we could find no evidence of temporal variation in MHC allele frequency. The Greater Flamingo colony in France was sampled 9–11 years prior to other breeding colonies in our study. The slow reproductive rate of flamingos means that this represents approximately a difference of a single generation with other breeding colonies . Indeed, Pradel et al.  demonstrated that at age 10, virtually all individuals had attempted to breed at least once and about 90% of them have already done so at age 9. Thus our results tentatively suggests that allele frequency does not significantly shift during this short time period as reported in species with faster reproductive rates . However more generations from the same geographical locality are needed to confirm the latter. Nonetheless, in the case of Greater Flamingos in the Mediterranean basin, given the lack of spatial MHC population structure, high allelic MHC diversity appears to be maintained by overdominant heterozygote advantage and/or population wide frequency dependent selection rather than local fluctuating selection and/or local variation in frequency dependent selection.
The lack of locally adapted alleles may be explained by gene swamping, whereby specific local polymorphism is lost because gene flow is larger than selection . Furthermore, for local population structure at the MHC to occur there needs to be strong spatial variation in parasite communities . While Greater Flamingos occupy a large diversity of wetland habitats across the Mediterranean, the parasite communities that flamingos encounter remain by and large unknown. However, a strong driver of MHC class IIB are likely to be the multiple species of helminths that Greater Flamingos are known to be parasitized by , with artemia species consumed by Greater Flamingos known to be the intermediate host . Indeed parasitic helminths are known to cause significant pathology in birds which can result in lower fat content , increased chick mortality  and lower reproductive success . Furthermore, migratory species are known to harbour a larger diversity of nematodes [14, 15], in particular species with wide geographic distributions and using multiple aquatic habitats  such as is the case for Greater Flamingos. It is therefore possible that flamingos do encounter a strong variation of parasites at different sites in the Mediterranean, but significant breeding dispersal [22, 70] leads to sufficiently strong gene flow to disrupt any possible local adaptation. In this case, dispersal may also enable Greater Flamingos to escape from locally prevalent pathogens that are highly adapted to the hosts.
Alternatively, the high dispersal rates of flamingos may act to facilitate the dispersal of parasites themselves, and, hence, homogenise parasite communities across the Mediterranean. Furthermore it has been suggested that saline wetlands may harbour fewer parasites than freshwater wetlands . For example, Mendes et al.  found that species of wading birds inhabiting saline habitats had fewer blood parasite infections than species from freshwater habitats. Indeed, despite the screening of chicks, studies have never detected blood parasites in Greater Flamingos in the Mediterranean [56, 73]. Regarding helminths, a comparative study by Poulin  using data from 389 parasitic species of cestodes, trematodes and nematodes in 158 bird species found that a positive correlation between heavy infections in local host populations and the ability to exploit many host species. The latter is suggestive that helminth infections that affect most fitness may be widespread rather than locally constrained. Therefore, the diversity and heterogeneity of parasites encountered by flamingos may be comparatively low and, in turn, the cost of dispersing, in terms of encountering new parasites that they are not immune to, may also be comparatively lower than for inland species. Indeed, the high dispersal rates encountered in Greater Flamingos are on par with the ones found in marine colonial birds that tend to have a higher dispersal rates than other migratory avian species (reviewed in ). In the absence of local variation in parasite communities, the benefits of dispersing, such as finding alternative breeding and feeding sites to avoid high competition at the natal site (especially for young inexperienced birds), may outweigh the costs.
Regardless of whether parasites vary across time and space in flamingos, the very high diversity of MHC Class IIB alleles observed in this study suggests strong pathogen-mediated balancing selection at the scale of the whole population. Given the lack of local MHC population structure, MHC diversity appears to be maintained by overdominant heterozygote advantage and/or population wide frequency dependent selection rather than local fluctuating selection and/or local variation in frequency dependent selection. Further understanding of how pathogens vary across space and time will be crucial to further elucidate the mechanisms maintaining MHC diversity in large panmictic populations with high dispersal rates .
We are deeply grateful to the late Luc Hoffmann and the late Alan Johnson for the instigation of the long-term study on the Greater Flamingo. We warmly thank all the people who participated in collecting genetic samples of Greater Flamingos and in particular Christophe Germain and Antoine Arnaud. We thank Christine Dubreuil and Maria Teixeira for laboratory assistance. We thank Miguel Alcaide for kindly providing coot MHC genotype data for the rarefied analysis.
This study was funded by the TOTAL foundation, the Conseil Régional de Bourgogne, the MAVA foundation, the Centre National de la Recherche Scientifique (CNRS), and the German Science Foundation (DFG Gi 1065/2-1).
Availability of data and materials
MG, AB, SS and FC conceived/designed the study. MG conducted all laboratory work. FC, AB, MR., JA, BS and OO organised the field work and/or provided the samples used in this study. MG wrote the first draft of the paper and all authors contributed to subsequent versions. MG and AC analysed and interpreted the data. All authors gave final approval for publication and agree to be held accountable for the work performed.
The authors declare that they have no competing interests.
Ethics approval and consent to participate
Ringing and sample collection of greater flamingo chicks in France were authorised through the personal permit (number 405) of Alan Johnson and Arnaud Béchet delivered by the Centre de Recherche sur la Biologie des Populations d'Oiseaux (CRBPO, Muséum national d'histoire naturelle, France). The study protocol was reviewed and approved by the CRBPO. Ringing and sample collection of greater flamingo chicks in Turkey were authorised through the personal ringing licence (Certificate number = 1) of Ortaç Onmuş and ringing licences of various Turkish bird ringers (who have participated to the ringing studies) by the General Directorate of National Parks of the Ministry of Forestry and Water Works of the Republic of Turkey. All the studies of ringing and sample collection in Turkey were applied under the surveillance of and approved by the General Directorate of National Parks. Boudjéma Samraoui was authorised to sample and ring flamingo chicks through the Algerian Ministère de l'Enseignement Supérieur et de la Recherche Scientifique (MESRS). The Consejería de Medio Ambiente of the Junta de Andalucía (Regional Government) authorised the ringing and sampling of flamingo chicks in Spain.
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