Fig. 3

Conserved non-coding sequences (CNS) and transcription factor binding sites (TFBS) in AgGPRop3 and AgGPRop4. a Anopheles gambiae opsin genes showing 5′- and 3′-non coding region on chromosome (Chr) 2R. b Alignment between the 5′ region of the AgGPRop3 (2,612 bp) and of AgGPRop4 (10,000 bp) was submitted to mVISTA using the LAGAN algorithm to identify CNS. Conserved regions are shaded (red). The minimum value on the Y-axis is 50%, the minimum conservation identity is 70%, and the minimum length for a CNS is 100 bp (default values on the VISTA plot). Because VISTA calculates the percentage of conservation identity as the number of matches divided by the length of the reference sequence (not the length of the alignment), the length in bp of the CNS represents the reciprocal alignment value. c Sequences were submitted to rVISTA to identify conserved clusters of the 50 known TFBS in insects using TRANSFAC®. Vertical (green) lines indicate the position of the 25 conserved TFBS identified, which occur in the clusters 1 and 2