Fig. 3

Arrangement of amino acid motifs in MiSp sequences of cobweb and orb-web weaving spiders. Latrodectus and S. grossa accession numbers are in Table 1. Lh, L. hesperus; Lt, L. tredecimguttatus; Lg, L. geometricus; Sg, S. grossa; Pt, P. tepidariorum based on combining the incompletely assembled MiSp-encoding region from Scaffold 853 of the i5K genome with an ~2.3 kb TOPO-cloned PCR product, KX584004; Av, A. ventricosus, JX513956.1 [30]. Lengths of missing amino acid sequences in Pt MiSp_v1 are based on the length of gaps in Scaffold 853 of the i5K genome, assuming no introns are present within those gaps. The last gap was predicted to be 1782 bp long (594 aa if no intron) in Scaffold 853, but our TOPO clone added 2236 bp, including a 1175 bp intron, which was longer than the predicted gap. The repetitive encoding region at the 3’ end of our TOPO clone did not overlap with the encoding region present in the i5K genome prior to the last gap