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Fig. 5 | BMC Evolutionary Biology

Fig. 5

From: Surface layer proteins from virulent Clostridium difficile ribotypes exhibit signatures of positive selection with consequences for innate immune response

Fig. 5

SLPs induce phagocytosis at variable levels in macrophages. Phagocytosis of FITC-labelled fluorescent beads by J774 macrophages in the presence of LPS and SLP ribotypes 001, 014, 027 and 078. Cells were stimulated for 24 h with SLP (20 μg/mL) or LPS (100 ng/mL) and beads were then added (10 beads/cell). Percentage of the cell population was measured by the quantity of FITC signal from the cells using flow cytometry

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