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Table 1 Bacterial strains and plasmids used in this study

From: A passive mutualistic interaction promotes the evolution of spatial structure within microbial populations

Strain or plasmid

Relevant characteristics

Reference or source

E. coli strain

 BW25113 (∆proC)

BW25113 with ∆proC:Km R; KmR

[32]

 BW25113 (∆trpC)

BW25113 with ∆trpC:Km R; KmR

[32]

 DH5α/λpir

Used for replication of pUC18T derivatives; λpir80dlacZ ΔM15 Δ(lacZYA-argG)U169 recA1 hsdr17 deoR thi-1 supE44 gyrA96 relA

[34]

Plasmid

 pUC18T-mini-Tn7T-LAC-Gm

pUC18-based conditionally replicative delivery plasmid for mini-Tn7-LAC-Gm; ApR, GmR, mob+

[33]

 pUC18T-mini-Tn7T-LAC-Gm-egfp

pUC18T-mini-Tn7T-LAC-Gm containing egfp immediately downstream of P lac ; ApR, GmR, mob+, egfp+

this study

 pUC18T-mini-Tn7T-LAC-Gm-echerry

pUC18T-mini-Tn7T-LAC-Gm containing echerry immediately downstream of P lac ; ApR, GmR, mob+, echerry+

this study