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Table 1 Bacterial strains and plasmids used in this study

From: A passive mutualistic interaction promotes the evolution of spatial structure within microbial populations

Strain or plasmid Relevant characteristics Reference or source
E. coli strain
 BW25113 (∆proC) BW25113 with ∆proC:Km R; KmR [32]
 BW25113 (∆trpC) BW25113 with ∆trpC:Km R; KmR [32]
 DH5α/λpir Used for replication of pUC18T derivatives; λpir80dlacZ ΔM15 Δ(lacZYA-argG)U169 recA1 hsdr17 deoR thi-1 supE44 gyrA96 relA [34]
Plasmid
 pUC18T-mini-Tn7T-LAC-Gm pUC18-based conditionally replicative delivery plasmid for mini-Tn7-LAC-Gm; ApR, GmR, mob+ [33]
 pUC18T-mini-Tn7T-LAC-Gm-egfp pUC18T-mini-Tn7T-LAC-Gm containing egfp immediately downstream of P lac ; ApR, GmR, mob+, egfp+ this study
 pUC18T-mini-Tn7T-LAC-Gm-echerry pUC18T-mini-Tn7T-LAC-Gm containing echerry immediately downstream of P lac ; ApR, GmR, mob+, echerry+ this study