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Table 1 The number of initiated inbred DV lines, those used in the experiment, and those surviving until the current moment, grouped by their source populations in the Northern Adriatic Sea [41]

From: No evidence for strong cytonuclear conflict over sex allocation in a simultaneously hermaphroditic flatworm

Source (Site)

Initiated (n) (in 2004)

Used DV lines (in 2006)

Surviving DV lines (currently)

Lignano Sabbiadoro 1995 (P1)

30

22f, 39g, 57g

18g, 22f, 27g, 39g, 41g, 57g, 71e, 83g

Lignano Sabbiadoro 2002 (P1)

7

na

25d

Isola di Martignano 2002 (X)

8

47i

47i

Isola di Martignano 2003 (PS)

90

26c, 28c, 33c, 49j, 67a, 68b, 72, 81c, 84j

16c, 26c, 28c, 33c, 49j, 51c, 65c, 67a, 68b, 81c, 84j

Bibione 2003 (UV)

105

1c, 3d, 6d, 8d, 12d, 13c, 14, 29c, 35d, 40, 44d, 46d, 50d, 61d, 69d, 75h, 76

1c, 3d, 6d, 8d, 10c, 12d, 13c, 20g, 29c, 31c, 35d, 37c, 44d, 46d, 50d, 61d, 69d, 75h

Total (n)

240

30

39

The letters indicate the different mitochondrial haplotypes of DV lines, with the following polymorphic bases and resulting amino acid substitutions (base positions are given with respect to the start of the sequences for each fragment):

Haplotype

350

373

408

421

587

40

127

148

386

441

528

a

A

C

C

A

G

A

A

C

T

C

T

b

A

C

C

T

G

A

A

C

C

C

T

c

A

C

C

T

G

A

A

C

T

C

T

d

A

C

T

T

G

A

A

C

T

C

T

e

A

T

C

A

A

A

A

C

T

C

T

f

A

T

C

A

G

G

A

C

T

A

C

g

A

T

C

A

G

G

A

C

T

C

C

h

A

T

C

A

G

G

A

C

T

C

T

i

A

T

C

T

G

A

A

T

T

C

T

j

G

C

C

T

G

A

G

C

T

C

T

Encoded amino acids

I/V

F

A/V

L/F

V/I

L

W

R

F/S

F/L

V

Open reading frame

nad2

nad2

nad2

nad2

nad2

cox1

cox1

cox1

cytb

cytb

cytb

  1. Underlined DV lines were used in the experiment but are no longer available. Note that DV stands for Dita Vizoso, who was mainly responsible for their establishment. The table footer indicates the observed haplotypes in the sequenced genes (see also main text)