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Table 1 The size of N2 worms with or without JU1410-derived pheromone with (A) live or (B) dead E. coli OP50, showing the mean length (SD = standard deviation), and t-test results comparing the pheromone-treated and control worms (significant effects are in bold), and (C) the larval stage of development after 36 h of growth as judged by the development of the vulva as in (A) showing the number and, in parentheses, the proportion at each stage

From: Pheromone modulates two phenotypically plastic traits – adult reproduction and larval diapause – in the nematode Caenorhabditis elegans

 

Without pheromone

With pheromone

t-test

Time (h)

Length (μm)

SD

Length (μm)

SD

d.f.

t

P

(A)

0

284.89

42.87

287.63

26.70

nd

nd

nd

12

344.45

17.54

342.08

27.65

33

0.34

0.74

24

573.36

76.46

560.87

45.07

33.4

0.67

0.51

36

683.58

51.07

804.24

65.86

50.5

−7.86

<0.001

48

931.95

143.34

1061.76

106.71

43.3

−3.93

<0.001

60

1050.69

77.81

1282.13

84.13

74.8

−12.54

<0.001

72

1269.93

50.59

1399.32

95.65

57.4

−7.28

<0.001

96

1351.72

50.76

1496.66

113.61

50.7

−7.03

<0.001

120

1426.73

144.42

1524.65

116.55

30.6

−2.16

0.04

168

1399.29

119.21

1538.65

146.62

23.2

−2.73

0.01

(B)

12

369.8678

24.67

370.37

25.53

67.4

−0.08

0.94

24

454.81

33.69

470.23

31.05

74.8

74.79

0.04

36

685.24

91.94

713.83

71.71

78.6

78.59

0.12

48

946.89

79.87

961.37

81.85

84.0

83.95

0.41

60

1126.54

66.14

1127.45

92.39

67.88

−0.05

0.96

72

1273.73

70.68

1316.78

57.96

80

−3.03

0.003

96

1298.74

49.27

1311.07

39.78

88.47

−1.36

0.18

120

1402.26

55.53

1412.51

67.68

93.07

−0.83

0.41

168

1506.47

72.66

1493.70

77.99

97.51

0.85

0.40

(C)

Developmental stage

Without pheromone

With pheromone

Early L3

1 (0.04)

0 (0)

Mid L3

0 (0)

0 (0)

Late L3

1 (0.04)

1 (0.04)

L3/L4 moult

3 (0.12)

0 (0)

Early L4

9 (0.36)

1 (0.04)

Mid L4

5 (0.2)

6 (0.23)

Late L4

4 (0.16)

14 (0.54)

Late L4 to adult

2 (0.08)

4 (0.15)

Total

25 (1)

26 (1)

  1. L is larval stage. nd = done